DreEX6067863 @ danRer10
Exon Skipping
Gene
ENSDARG00000077582 | ank3b
Description
ankyrin 3b [Source:ZFIN;Acc:ZDB-GENE-060621-2]
Coordinates
chr12:7654407-7665541:-
Coord C1 exon
chr12:7665418-7665541
Coord A exon
chr12:7658440-7658542
Coord C2 exon
chr12:7654407-7654513
Length
103 bp
Sequences
Splice sites
3' ss Seq
ATTAATGTAAATGCCAACAGCCT
3' ss Score
1.82
5' ss Seq
ACGGTATGC
5' ss Score
8.56
Exon sequences
Seq C1 exon
GTGAGGATGCGATGACCGGAGACACAGACAAATATCTGGGGCCGCAGGATCTGCGTGAGCTCGGAGACGACTCTCTGCCTCAGGAAGGATATGTTGGATTTAGTGTTGGAGCCCGAACGGCCAG
Seq A exon
CCTGCGCTCCTTCAGTTCGGATAGGTCCAACACTCTCAACCGGAGCTCCTTCGCCCGCGACAGCATGATGATCGAAGAGATCCTGGCGCCCACCAAGGACACG
Seq C2 exon
CACCTGGCCTTAGCGAAAGATTACAACACGGACTCAATGAGACGTTACAGCTGGACGCCAGATACTATGGACAATGTGAACCTGGTCTCCAGTCCTGTCCATTCAGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000077582-'35-33,'35-30,38-33=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.551 A=0.208 C2=0.087
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
PF0079115=ZU5=PU(1.0=2.8)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGAGGATGCGATGACCGGAG
R:
AATGGACAGGACTGGAGACCA
Band lengths:
226-329
Functional annotations
There are 1 annotated functions for this event
PMID: 26024478
The paper describes and annotates all exons to an extreme detail. Wild-type isoform, but not the variants (either skipping 31 or 28-31), bind beta-spectrin. Exon 28 (MmuEX0004926), exon 29 (MmuEX6048924), exon 31 (MmuEX6048923)
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]