Special

MmuEX0004926 @ mm9

Exon Skipping

Gene
ENSMUSG00000069601 | Ank3
Description
ankyrin 3, epithelial [Source:MGI Symbol;Acc:MGI:88026]
Coordinates
chr10:69390443-69413806:+
Coord C1 exon
chr10:69390443-69390566
Coord A exon
chr10:69395142-69395244
Coord C2 exon
chr10:69413700-69413806
Length
103 bp
Sequences
Splice sites
3' ss Seq
GGTGACTGTAAATACAACAGCCT
3' ss Score
-0.19
5' ss Seq
CAGGTAAGC
5' ss Score
9.88
Exon sequences
Seq C1 exon
GTGAAGATGCCATCACAGGGGACACTGACAAGTATCTCGGGCCACAGGACCTTAAGGAGCTAGGTGATGACTCCCTGCCAGCAGAAGGTTACGTAGGCTTCAGTCTTGGAGCCCGTTCTGCCAG
Seq A exon
CCTCCGCTCCTTCAGTTCGGATAGGTCCTACACCTTGAACAGAAGCTCCTACGCAAGGGACAGCATGATGATAGAGGAACTTCTGGTACCATCCAAAGAGCAG
Seq C2 exon
CACCTGACGTTCACGAGGGAGTTTGATTCTGACTCCCTCAGACACTACAGTTGGGCAGCGGACACGTTAGATAATGTGAACCTGGTCTCAAGCCCGGTGCATTCTGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000069601_MULTIEX1-25/25=24-C2
Average complexity
S*
Mappability confidence:
100%=100=100%
Protein Impact





	

	
ORF disruption upon sequence exclusion





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.219 A=0.000 C2=0.011
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
NO

							
A:
NO

							
C2:
PF0079115=ZU5=PU(1.0=2.8)

						

					

				








    
Main Inclusion Isoform:
ENSMUSP00000090090f5603A





     
                   









    
Main Skipping Isoform:
ENSMUSP00000045834





     
      









    
Other Inclusion Isoforms:
ENSMUSP00000061698, ENSMUSP00000090087, ENSMUSP00000090088, ENSMUSP00000090089, ENSMUSP00000090090, ENSMUSP00000090090f5390A, ENSMUSP00000090090f5391A, ENSMUSP00000090090f5392A, ENSMUSP00000090090f5393A, ENSMUSP00000090090f5394A, ENSMUSP00000090090f6290A, ENSMUSP00000090090f6291A
          









    
Other Skipping Isoforms:
NA
  



Associated events






Other assemblies


mm10




Conservation


Human
(hg38)
chr10:60134271-60134373 
ALTERNATIVE
HsaEX6088523
(ANK3)
Human
(hg19)
chr10:61894029-61894131 
HIGH PSI
HsaEX6088523
(ANK3)
Rat
(rn6)
chr20:20385503-20385590 
ALTERNATIVE
RnoEX0009395
(Ank3)
Cow
(bosTau6)
chr28:15929831-15929933 
Chicken
(galGal4)
chr6:8447975-8448077 
Chicken
(galGal3)
chr6:10108886-10108988 
HIGH PSI
GgaEX6039829
(Q90717_CHICK)
Zebrafish
(danRer10)
chr12:7658440-7658542 
HIGH PSI
DreEX6067863
(ank3b)
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
GTGAAGATGCCATCACAGGGG

				
R: 
GAATGCACCGGGCTTGAGAC

				
Band lengths: 
228-331

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 1 annotated functions for this event 


PMID: 26024478
MmuEX0004926
The paper describes and annotates all exons to an extreme detail. Wild-type isoform, but not the variants (either skipping 31 or 28-31), bind beta-spectrin. Exon 28 (MmuEX0004926),  exon 29 (MmuEX6048924), exon 31 (MmuEX6048923)


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types






Retrieved from "https://vastdb.crg.eu/wiki/Special:VastDB"