DreEX6069944 @ danRer10
Exon Skipping
Gene
ENSDARG00000009341 | nrap
Description
nebulin-related anchoring protein [Source:ZFIN;Acc:ZDB-GENE-060616-224]
Coordinates
chr12:30485179-30486615:+
Coord C1 exon
chr12:30485179-30485286
Coord A exon
chr12:30486011-30486208
Coord C2 exon
chr12:30486511-30486615
Length
198 bp
Sequences
Splice sites
3' ss Seq
TTAAAATTTCTTTAAAATAGGTT
3' ss Score
7.77
5' ss Seq
GACGTAAGT
5' ss Score
10.93
Exon sequences
Seq C1 exon
ATTAAATATAAGGAGTCCTATACAAAGAATAAGGACAAAGCCATTGGAATGGATGTGAGTGACTCCAAAACACTGCACTCCCTTCAGGTGGCCAAGCTAAGCAGTGAG
Seq A exon
GTTGCTTATAAAAAGGATTCTAAGGAAACTCAATCCAAGTGCCACCTGCCTCAGGACATGGTGAATCTGAGTCATGCTAAGAAGGCTCAAGCTCTGGCCAGTGATCTGGAATATAGGAAGAAGTTACATGATTATACTCTCCTGTCTGATGACATGAAAGTGCAGCAAGCTAAAAAAGCCTACAAACTGCAGAGTGAC
Seq C2 exon
AACCAGTACAGATCAGACCTGAACTGGATGAAAGGAGTTGGATGGGAGGCCGATGGATGCCTAGATGTAGAACAGGCCAAGAAAGCAGGAGAACTCTTCAGTGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000009341-'20-24,'20-23,21-24=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.028 A=0.167 C2=0.000
Domain overlap (PFAM):
C1:
PF0088013=Nebulin=PD(37.9=30.6)
A:
PF0088013=Nebulin=WD(100=39.4),PF0088013=Nebulin=PU(55.2=24.2)
C2:
PF0088013=Nebulin=PD(37.9=31.4),PF0088013=Nebulin=PU(55.6=42.9)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGAGTCCTATACAAAGAATAAGGACA
R:
CTCACTGAAGAGTTCTCCTGCT
Band lengths:
202-400
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]