DreEX6073699 @ danRer10
Exon Skipping
Gene
ENSDARG00000028533 | macf1a
Description
microtubule-actin crosslinking factor 1a [Source:ZFIN;Acc:ZDB-GENE-030131-3606]
Coordinates
chr19:35568499-35570609:-
Coord C1 exon
chr19:35570450-35570609
Coord A exon
chr19:35570286-35570369
Coord C2 exon
chr19:35568499-35568606
Length
84 bp
Sequences
Splice sites
3' ss Seq
TTCAGTGTCTTCTCCTGCAGGCA
3' ss Score
10.09
5' ss Seq
AAGGTACAG
5' ss Score
8.04
Exon sequences
Seq C1 exon
TCAAGCGGATCTGGAGATGCTGGGATCTGTTCTGCAGCAGTATAGAGAAGGCCACACTTCTCTCATACGCTGGATAGAGGAGACCACCGAAAAACAGGAGAACACACAGCCCGAACAAACTGACAGCAGAGCTTTGTCAGAACAGCTCGCACAGCAAACT
Seq A exon
GCATTGGTAGTTGAGATTGAACAGAACCAGGTCAAACTGGATGAGTGCCAGACCTGCTCTAAACAGTACAGCGCTGCCGTTAAG
Seq C2 exon
GACTATGAACTTCAACTTATGACGTTCAGAGCATTTGTTGAATCAACCCAGAAGTCCCCTGTGAAAAGGAGGAGGATGCACTCTTCCTCAGATGTCATCACACAGGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000028533-'35-41,'35-40,36-41=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.426 A=0.000 C2=0.277
Domain overlap (PFAM):
C1:
PF0043516=Spectrin=PD(5.3=11.1)
A:
NO
C2:
PF086146=ATG16=PU(3.5=17.1)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACGCTGGATAGAGGAGACCAC
R:
GGAAGAGTGCATCCTCCTCCT
Band lengths:
181-265
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]