DreEX6095169 @ danRer10
Exon Skipping
Gene
ENSDARG00000014527 | pdk3a
Description
pyruvate dehydrogenase kinase, isozyme 3a [Source:ZFIN;Acc:ZDB-GENE-061220-3]
Coordinates
chr24:24799178-24802941:-
Coord C1 exon
chr24:24802834-24802941
Coord A exon
chr24:24801371-24801484
Coord C2 exon
chr24:24799178-24799459
Length
114 bp
Sequences
Splice sites
3' ss Seq
CTCCATAACCTGATCTGTAGGCT
3' ss Score
4.14
5' ss Seq
AAGGTAAAA
5' ss Score
8.38
Exon sequences
Seq C1 exon
ATCAGTGACAGGGGAGGGGGCGTGGCATTACGGAAGATCGACAGGCTATTTAACTACACCTACTCGACTGCACCCACACCCAGTCTGGACTCCAAACGAGTGCCGCTG
Seq A exon
GCTGGGTTTGGCCATGGTTTGCCGATCTCAAGACTCTACGCCCGATACTTTCAAGGAGACCTGAAACTGTACTCGATGGAAGGTGTGGGGACTGATGCCGTCATCTATTTGAAG
Seq C2 exon
GCCCTCTCCAGTGAATCATTTGAGCGTCTGCCCGTCTTCAACAAATCTGCATGGCGTCACTACCAAGGAGGCCCAGGAGCAGACGACTGGAGCAACCCGAGCAAAGAGCCCAGAGATACCTCCAAATACAAGGCCAAGAGATAATTGCACTCACCCCATCCAAACACCCATAACACTCCCTCTGTCTTTAGTCCAGCTCATCGATGCTGTGTATTCGATACTTGTTAGCATTTCCAGAGCAGAAGTTTCTTTACATCTCTGCTTCATCCTCCTTTGTGCAGC
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000014527-'11-14,'11-13,12-14=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.489
Domain overlap (PFAM):
C1:
PF0251821=HATPase_c=FE(28.0=100)
A:
PF0251821=HATPase_c=FE(29.6=100)
C2:
PF0251821=HATPase_c=PD(0.8=2.1)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACACCCAGTCTGGACTCCAAA
R:
CACAGCATCGATGAGCTGGAC
Band lengths:
244-358
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]