DreINT0025725 @ danRer10
Intron Retention
Gene
ENSDARG00000001431 | actn3b
Description
actinin alpha 3b [Source:ZFIN;Acc:ZDB-GENE-030131-2040]
Coordinates
chr7:6889837-6891394:+
Coord C1 exon
chr7:6889837-6889902
Coord A exon
chr7:6889903-6891235
Coord C2 exon
chr7:6891236-6891394
Length
1333 bp
Sequences
Splice sites
5' ss Seq
CTGGTGAGT
5' ss Score
10.1
3' ss Seq
GTCTGGTTGTGTTCTGACAGGGT
3' ss Score
7.54
Exon sequences
Seq C1 exon
AAGAGAAACGGCATGATGGACCCCGACGACTTCCGCGCCTGTCTGATCTCTATGGGTTACGATCTG
Seq A exon
GTGAGTGACAGCAAAGCCCCGTCCCTTTGTATGAAGCCACACACTTCTTTGAAAAACACGCATCTTTTACATGTCTGCTAAATATGTTTTATAGTTTGTGTTTTCCATAGCAGTATATTAACAATGATGCATTTAAAGGGCACCTATTATGCTTAAATTCACTTTTATAAGTGGTTTAAACACAGTTGTTTGGTGACAGTGTGTAAATATAACCAATATAATCTTTGTTGTATTCACACTTGATTAAAACAGTCTGCAGAAACACTTTGATTGACATTCTCCCTTTGTACGTGTCATCAGAATAGAAAAGACCCGCCCACTAGTGACCATCTCTCTCTCATTAGCATAAACATCCCCTGAGTAAAAAGCAGCCATTCACCCTTTGAGTTTTGAATCTGCCACTATGCCGACACACAGGTATTTGTAGCTCCGCTCTCTTTTGAAAAGGGCACAATCTCAATTGAATTTAAAGCGACAGTCACCAAAATGCCTATTGGCTTACAGCAGGAAAGTCCAAACTCGGTCCAGTGTCCTGCTGAGTTTAGCTTCAACACACCTGCCAGGACGTTTCTAGTATATCTAGTAAGAGCTTGATTAGCTGGTTCAGGTGTGTTTGATTAGGGTTGGAGCTAAACTCACCAGGACACCGGCCCTCCAGGACTGAGTGTGGACACCCTTGGCTTAAAAAGAATAGTCACTGCAGTAAAAGTTGACCTGTTCTGCTGATTTCTACCTCTGTTTCAAAAGAAACATTTGTTGTTTATTTATATTTTGATATTTCATCAAATTGTTCTGCTTAATACCTTATTATCTGCATGTTTATGCTGATTTCATGATGTGAGTATAATAGAGTATGAGTCTGTAATTTAGCTTTTTTGTTATTTGTATTTGGAGTTTTAAAAAAGTTTTACTGTTTAAAAACAAAATTTGTTTTTCATTTAAGGTAAAAAAATGTCAGAATTAAATATTATTAAACTCGATATGTGGCTTTTGCTTTTTTTAAAACAAATACTTTTATACATAAATACCCTGATTTAAGTTTTTACAAATTTATAATAAGCAAAATGTCTAATTAAAAAAAAAAACATTTCATTTTAATCCGATTAATCAAAAAAAAAATCAGCCGATTAATTTATTAACAGACTAAACATTAGTTGCAGCCCTAAACTCAACTGATTTGAATGGCCACACATTTGAATTGATTTTCAACAACCTGGCTGTTCATCATTACATCCCCGACCAACAGTCATTTTAAAGATGTTTTCTTAAGACTAGCAAAATGAACATGTCTCTTTAACCTCATTGGCTGTTTGGTCTGGTTGTGTTCTGACAG
Seq C2 exon
GGTGAGGTGGAGTTCGCCCGCATTATGACCCTGGTGGACCCCAACAACACCGGCGTGGTGACCTTCCAGGCCTTCATCGACTTCATGACCCGCGAGACTGCCGAAACCGACACTGCTGAACAAGTCATGGCCTCCTTCAAGATCCTGGCCTCCGACAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000001431:ENSDART00000001649:19
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (disopred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF134991=EF-hand_7=FE(26.9=100)
A:
NA
C2:
PF134991=EF-hand_7=PD(35.9=52.8),PF087265=EFhand_Ca_insen=PU(26.9=34.0)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GAGAAACGGCATGATGGACCC
R:
CTTGTCGGAGGCCAGGATCTT
Band lengths:
223-1556
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]