DreINT0034169 @ danRer10
Intron Retention
Gene
ENSDARG00000033361 | asl
Description
argininosuccinate lyase [Source:ZFIN;Acc:ZDB-GENE-040426-1152]
Coordinates
chr21:31221316-31221581:+
Coord C1 exon
chr21:31221316-31221413
Coord A exon
chr21:31221414-31221503
Coord C2 exon
chr21:31221504-31221581
Length
90 bp
Sequences
Splice sites
5' ss Seq
AGCGTAAGA
5' ss Score
6.21
3' ss Seq
ATTTTTTTTAATCCTTTTAGAGA
3' ss Score
8.12
Exon sequences
Seq C1 exon
GTTGCAACTGATATGCGACTCTGGTTACGGGATGGTATTGCCACGCTTAAAGAACTGGCTTTGCAACTCATCAACACTATGGTGGAAAGAGCTGCAGC
Seq A exon
GTAAGAATATGCACAGTAATCTTAATACATTTATTAATAAGAGTAATGTACTATTGCCAAATACATAGCTATTTTTTTTAATCCTTTTAG
Seq C2 exon
AGAGATTGAAATCCTGTGCCCAGGATACACTCACATGCAGAGAGCCCAGCCAATCAGATGGAGTCACTGGCTTCTCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000033361:ENSDART00000040190:5
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0020615=Lyase_1=FE(10.8=100)
A:
NA
C2:
PF0020615=Lyase_1=FE(11.4=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGCAACTGATATGCGACTCTGG
R:
GCCAGTGACTCCATCTGATTGG
Band lengths:
167-257
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]