DreINT0038451 @ danRer10
Intron Retention
Gene
ENSDARG00000099069 | bsk146
Description
brain specific kinase 146 [Source:ZFIN;Acc:ZDB-GENE-060313-3]
Coordinates
chr12:543658-546042:-
Coord C1 exon
chr12:545840-546042
Coord A exon
chr12:545620-545839
Coord C2 exon
chr12:543658-545619
Length
220 bp
Sequences
Splice sites
5' ss Seq
CAGGTAATG
5' ss Score
9.43
3' ss Seq
GTCGATGTTGTGGTGTGCAGGTG
3' ss Score
6.49
Exon sequences
Seq C1 exon
GTAGCAAAATGGCTCTGAAGTTTCTGAAGAAGAAAAGCACCAAGCTGAAGAGCTTCTTGCGGGAGTACAGCATCTCTCTCTATCTCTCTCCATGTCCCTTCATCATCAACATGTTCGGCATCGCCTTTGAGACAGACGAGTATTATGTGTTTGCGCAGGAATACGCTCCTTCTGGGGACCTGTTTGACATCATTCCACCACAG
Seq A exon
GTAATGCAGTGCTGTTTAAGCAGAGTAATGCACATCAGTAAAGTCAGAAATCAACTTTTTAAAAGTATTATTATTGGTTTAGGTCACAATTCATGCTATTAACTGCTGGATTATTGCCTGTAGCAAATTAGGCAATATAAAGCCATGGATCTGGCAACCTTTGCGCTGAGAGCGATGCCTATGTTTGATTACTGATTATTGTCGATGTTGTGGTGTGCAG
Seq C2 exon
GTGGGTCTGCCAGAGCCGGTGGCGAAGCGCTGTGTGCATCAGGTGGCCATCGCTCTCGAATACCTGCACTCCAAAAAACTGGTGCACCGAGACATCAAACCCGAGAACATCCTAATCTTCGACAAGGAGTGCCGTAAGGTCAAGCTGTCAGACTTCGGGATGGCGCGGCGCGCCGGTTCACCGGTCAAGCGTGTTAGCGGCACAATCCCATACACGGCTCCAGAACTCTGCGATACGTCCAAACACGACGGCTTCTGCGTGGACTACAGCACCGATGTGTGGGCGTTCGGAGTGCTGCTGTTCTGCATGCTAACCGGGAACTTTCCATGGGAAAAAGCGATGCCGTCTGATACTTTCTACGAGGAGTTTGTGCGCTGGCAGAAGCGGCGGACGGGTGCGGTTCCGTCACAATGGCGTCGATTTACAGACGAATCCCTGCGCATGTTTCGAAAGCTCCTAGCATTGGAGCAGGAACGCAGATGCAGCGTCAAGGAGGTTTTCGCACATTTAGGCCACCGCTGGATGCTTGATGGAACTAGCGGAAACCATCATCAATCCGTGCTCAACTCTTCATCAGAGGAAGACGAATTGCTTGTGGATCGTATGAAGCAGCAGACCTTATCTCCGACAGCTAACACGAGCAATGCTATTGAGCCCGGATCGGCTAACCACTTCACATCTGTGTCCACCAATAGCTCGGTGTCTTCCACTAACAGCTATGAGCGTTCGGCTAGAGACAGTCCTCCGACTAGCAGGATCCTGGTCACGACGCCTATAGAAATCTGCGTCTAAATGCACAGCTGATGAAATTAACATGCGCACAAGCTGACGCACACTCCTGTGATACGAGTTCTGCTGGATATTTAGCGCTGTTAAGGTGTGGTCACGTTAGCGGAACATCAGCTACATTTCACACACTACACATATCTCGATTTCAAACGATCTCTGTTGGTTTGCATGATATTTCTGAGACAAAAAGGTTTGTTTTGATACTAAACTCGTTACTTTCGTTAGTTGGAAATATGTGTCAGCCTACATTTTTACGAAAGTTGAAATACGTTGCTTTGGGTATGTTTTGTTGTACATTTCAGATTACTGATCCACTAGAGGGCGCTGTTTACATTTTTGCAAATCTGATATACCTTACTTTTAGTATGTTTTACTGTACATTTCCGATTACAAATCCACTAGAGGGCGCTGTCGACATTTTTGCAAATCTGTAATACGTTACTTTTGGTATGTTTTGTTGTACGTTTCAGATTATACATCCACTAGGGGGCGCAGTCTACATTTTTGTGAATCGAAAATACGTTGTTTAGGGTATATTTTGTGGTACATTTCAGATTACAAATCCACTAGAGTGCGCTGTCCACATTTTTGCAGATCTGAAAACGTTACTGAAGGTGTGTTTTGTTGTACGTTTTAGGTGACAAATCCACTAGAAGACGCAGTTACATTTTTGCAAATCTAAAATATGTTGATCAGAGTAAATTGTGTAGTACATTACAGATTACAAATCCATTAGAGGGCGCTGTCCATGTTTTGGCTAATCTGAAAAACCTTAGTTAGGGTATGTTTTGTTTTACGTTTAAGGTGACTAATCCACTAAAGGGCGCTATTTACATTTTCGTGAATTTTAAATACGTTACCTCTGAAGGTTGTGTTTTACATTTCAGATTACAAATCTAATAGAGGACGCTGTTTACTTTTTGTGCGAAACAGAAATACATTACTTAACGTATGTTTTGTTGTACATTTCTTATGACATACCCACTAGAGGGCGATGTCTACAACTTCATGCATCTGAAATGTTGATCTGAGTAAGTTTTGTAGTACATTTCAAAAAATAAATTCACTAGAGGGCACAGTCTACTGTCCATATTTTTGTAAATATAAAATGCATTCCTACAGGTAGATTTTTGTTGTACGTTAAGGTGACAAATCCACTAGAGGGCACTGTCT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000099069:ENSDART00000158355:3
Average complexity
IR
Mappability confidence:
NA
Protein Impact
Alternative protein isoforms
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.224
Domain overlap (PFAM):
C1:
PF0006920=Pkinase=FE(25.6=100)
A:
NA
C2:
PF0006920=Pkinase=PD(64.9=64.4)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CGCAGGAATACGCTCCTTCTG
R:
CGTGTTTGGACGTATCGCAGA
Band lengths:
297-517
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]