DreINT0041159 @ danRer10
Intron Retention
Gene
ENSDARG00000055592 | capn2b
Description
calpain 2, (m/II) large subunit b [Source:ZFIN;Acc:ZDB-GENE-050522-84]
Coordinates
chr22:26322064-26324343:-
Coord C1 exon
chr22:26324210-26324343
Coord A exon
chr22:26322233-26324209
Coord C2 exon
chr22:26322064-26322232
Length
1977 bp
Sequences
Splice sites
5' ss Seq
TAAGTGAGT
5' ss Score
6.43
3' ss Seq
GGTTTTATGGTTTCTGGCAGGGT
3' ss Score
6.23
Exon sequences
Seq C1 exon
TTCTGGCAGTTTGGCGAGTGGGTGGATGTGGTCATTGACGACCGGCTGCCGGTGAAAGATGGCGAGCTGTTGTTTGTTCACTCCGCTGAGAAAAACGAGTTCTGGAGCGCCCTGCTGGAGAAGGCCTATGCTAA
Seq A exon
GTGAGTGTTTTTTTGTGTTATTTCTTGTGTTCGTGAACCACCTTTAATCACAGTAATTAAGTTAGAAAGTGGCCAAACCAGAAAGTTAACCTGCCATCTAAGAAAAAAAGCAGGGCTTTAGATGTTTCTATGGAAATTATTTGAGCTCAAACTTTGCATACATGATGAACAAGCTTCATAGGTGTTTTCCAGGAATATGGGTGTGTCTTTTTATGTATTATGTGTTTAATCCAGCGCATATAATAACTTTATGTTAGCTTTATTTTAAAAGTATTTAATATACCCTTGTTTTTTCCTGCCAGCTGAACACAAAATGTTAGCCACCTGTTGTTTGTTGATTTAGTTTTGTGTAAGAGTACGTTCACAGCTGTTGACCTTTTCATTTTGCTTCCCCATCATGTGAACTATGAGAAAGGCCCTGCCCATATGTAATAATGTCATGCATTTTTTTTGTAGGCCAACCAGTATGTTAGCATGACCTTGGTTCCTTTGACAAAAAAACCCTTTGTGTTAATATCAAAAATTGTGTGTGCAATTTCACATATTTGCAAGTGCTTTAACAATAATGCCATTGAATGGTATATTATACGTGTGGATAAACATGATAAAAATCTTGTGTTAACCACCACTATTGTTAACCCCCACTTATATGTCAGGAATATGACCAAAAAACCAGAATAAAGGAACTGAAAATGCTAAAATGCTGATTTTGTCAGACAAAAAATGATCTCCACAGCAGTAATATTGCTGCTGGTGATCATTTCTACGTAATTTTTGCTTGATATTTTTCCTTTTCTGTGCTAAAATGCTTTATCTATATAAAATTTAGGCATTTAGAAATAAAAAAAAACCTCTCCAAAACCTTTAAAATCTACTAATACAAACATTTCACTTTAGTTTCTAGATGAAATAAGCACAGTACAAAACTAATATGTTATTTTTCTTACAAAGATGATCACATAGCACATAGCTGATTGTTTATGAACATGCCTTTTTTGTGCACCTTTTTGCATGTATAAATATATAAATGGAGAATATTAGTGCATGATATTGTATCTCAACATAGATTAATTTTCTTATGTAGTATGTTCAAAGCCACGAATAAACAAGATACATTTTTGTGGATGCGTTTTCACATTTGTTTTGTTAACACTTGTTTAGGTTTAGATCAGAGGTCATCAAACTTGTCCCTGGAGAGCTGGTGTCCTGCAGATTTTAGCTCCAACCCTAATCAAACACACCTGAACCAGCTAATCAAGGTAGTAGGTATACTTGAAACACCCAGGCTGTTGTGTTGAGGCAAGTTGGAGCTAAACACTGCAGGGTCACTGGCCCTCCAGGACCAGTATTTATGACCCCTGGTTTAGAGTATTTGAGTAGGTGATACAAGTATTGTTTCCAGGTGTTGCAGAAAAGTAGCCAATGTCACGTTTCCCTTGAGCCTATTTTGGCTAAAACATGTTTATAGTTGGTTATTTATCATGATTTAAGTAATAAACATTTGTTTTGTAGCATAAAAGATGCCATTTCATGAATGAGCTTCAGTTTTTCAACATTAAATGATCTGGAATTTGTGGCACGATGGCTTAGTGGTTTGCACTGTCGCCTCACAGCAAGAAAGGTGCTGGTTCGAGTCTCGGATGGCCTAGTTGGCATTTCTGTGTGGAGTTTGCATGTTCTCCCTGTGTTGGTGTGGGTGTCCTCTGGGTTCTCAGGTTTCCAGGAGTGTATGAGTGTTTCCCAGTAGAAAGGGCATCCGCTGTATAAAACAAATGCTGGAATAGTCGGTGGTTCATTCTGCTGTGGCAAATTAAAATAAGATTTTATTTCTGTGGGGTTTTATAATGGGGTTTTTAAAATCGTAAGTTAAATAAGTCTGTGGTAAAGAGAACTTGATAATATTTGTATCCTTGCCCTACAAACATGCGCGAACATGTAATTTCACTCTCTAACAGCAGGTTTTATGGTTTCTGGCAG
Seq C2 exon
GGTGAACGGCTGTTATGAAGCCCTGTCTGGAGGATCCACTAGCGAGGGTTTTGAGGACTTCACTGGAGGCATCGCTGAGAGCTACGAGATCAGAAAAGCACCGACCAATTTGTTTCAGATCATCCAGAAAGCCCTGGAGGCTGGAGCTTTGCTGGGCTGCTCCATCGAC
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000055592:ENSDART00000077958:4
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0064816=Peptidase_C2=FE(14.8=100)
A:
NA
C2:
PF0064816=Peptidase_C2=FE(18.8=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CAGTTTGGCGAGTGGGTGGAT
R:
GATGGAGCAGCCCAGCAAAG
Band lengths:
294-2271
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]