DreINT0045187 @ danRer10
Intron Retention
Gene
ENSDARG00000058000 | cep350
Description
centrosomal protein 350 [Source:ZFIN;Acc:ZDB-GENE-030131-6975]
Coordinates
chr8:14543347-14543995:-
Coord C1 exon
chr8:14543855-14543995
Coord A exon
chr8:14543481-14543854
Coord C2 exon
chr8:14543347-14543480
Length
374 bp
Sequences
Splice sites
5' ss Seq
GAAGTGAGA
5' ss Score
3.22
3' ss Seq
CCCCCCCCCTCTCCCCGTAGATG
3' ss Score
14.52
Exon sequences
Seq C1 exon
GTTCATGCAGAGCTGCAGGAGAGTTTGCTTCAGTCTCAACAGCAGGCGTTAGGAGGTTTGCATGAGGCCACAAACAAGATGATCAACCAGCAGGCTGAGGCCGCACGACACACAGCTGAAACAGCACGACACATTAAAGAA
Seq A exon
GTGAGACGTTATTACTCTTAAAACATTTGTATTAATTTTTTTAGGCTGAATAAAATGTATGTTTAAAAAAAGCTTTGAATGAAATTAACAAATGTGTTCTTGACAACCAAGTGAATTTAGCTGAAGTTACTTAAATTACAAACTAGAAACAAAACAAAAAAAAATCAACTAAAACTTTAAATAAAATAAATGAAAGTAATGTGTAGTTACAAACTCTTGGGTTCTTTATTGTAGAAAAAAAAACTACATTTTAGAAACAAGATTACATGCTTATTTGCTCAGTGTAAATTACACTTTTTCAAGGATTATACGTTTTGTTAATTCTCTCTCTCTCTGTCTCTACACCCCCCCCCCCCCCCCCCCTCTCCCCGTAG
Seq C2 exon
ATGACTGAGCTGGCGCGTTCACAGATTGTAGGAGCACTAAGTGTTCCTGGACCCTCCATTACAGCCCTGCATGACCAGCAGAGACAGCACAGCCGCAGTTCAAAACCGCACCAGACACAAACCCACACAGACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000058000:ENSDART00000146175:20
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.979 A=NA C2=1.000
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GAGCTGCAGGAGAGTTTGCTT
R:
CTGTGTGGGTTTGTGTCTGGT
Band lengths:
262-636
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]