DreINT0049955 @ danRer10
Intron Retention
Gene
ENSDARG00000012593 | col5a1
Description
procollagen, type V, alpha 1 [Source:ZFIN;Acc:ZDB-GENE-041105-6]
Coordinates
chr21:6782738-6782922:+
Coord C1 exon
chr21:6782738-6782791
Coord A exon
chr21:6782792-6782886
Coord C2 exon
chr21:6782887-6782922
Length
95 bp
Sequences
Splice sites
5' ss Seq
CCGGTAACA
5' ss Score
5.92
3' ss Seq
TGTGTTTTGCTTCTTTTCAGGGT
3' ss Score
12.02
Exon sequences
Seq C1 exon
GGTATTGCTGGACCCTCTGGACCTATCGGACCCATTGGACCTCCTGGACTCCCG
Seq A exon
GTAACATTTCTGCCTCCATTATATATTTATAGGGTAAATAAATATTTAAAGGTTAACTTCATCATGATCTATCTGTGTGTTTTGCTTCTTTTCAG
Seq C2 exon
GGTCCTCCTGGTCCTAAAGGAGCTAAAGGATCCAGT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000012593:ENSDART00000139598:58
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=1.000 A=NA C2=1.000
Domain overlap (PFAM):
C1:
PF0139113=Collagen=PD(17.9=66.7),PF0139113=Collagen=PU(30.4=94.4)
A:
NA
C2:
PF0139113=Collagen=FE(19.4=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGTATTGCTGGACCCTCTGGA
R:
GGATCCTTTAGCTCCTTTAGGACC
Band lengths:
87-182
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]