DreINT0053248 @ danRer10
Intron Retention
Gene
ENSDARG00000090428 | ctrb1
Description
chymotrypsinogen B1 [Source:ZFIN;Acc:ZDB-GENE-030131-1171]
Coordinates
chr7:34806364-34806805:+
Coord C1 exon
chr7:34806364-34806544
Coord A exon
chr7:34806545-34806671
Coord C2 exon
chr7:34806672-34806805
Length
127 bp
Sequences
Splice sites
5' ss Seq
ATGGCAAGT
5' ss Score
3.26
3' ss Seq
GCTGCCAATCTGTCCTTCAGCTC
3' ss Score
6.56
Exon sequences
Seq C1 exon
TCCATCAAGCATCCTAACTACAACAGCTTCACCATCAACAATGACATCCTGCTGATCAAGCTGGCCACTCCTGCTAAGATCAACACTCACGTGTCTCCTGTGTGTCTCGCCGAGACCAACGACAACTTCCCTGGTGGCATGAAGTGTGTGACCTCTGGATGGGGCCTGACCAGATACAATG
Seq A exon
GCAAGTATTATTGGATATAAAGAAGGCTCGATCTCTATCCTGAAATGTCTTCTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGGGGAAAGCTTTTAAAATGCTGCCAATCTGTCCTTCAG
Seq C2 exon
CTCCCGATACTCCTGCCCTGCTCCAGCAGGCCGCTCTGCCTCTGCTGACCAATGATGACTGCAAGCGTTACTGGGGAACCAACATCACTGATCTGATGATCTGCGCTGGAGCTTCTGGTGTCTCTTCTTGCATG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000090428:ENSDART00000037346:5
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0008921=Trypsin=FE(26.9=100)
A:
NA
C2:
PF0008921=Trypsin=FE(19.7=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGGCCACTCCTGCTAAGATCA
R:
AGAGACACCAGAAGCTCCAGC
Band lengths:
245-372
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]