DreINT0054232 @ danRer10
Intron Retention
Gene
ENSDARG00000013524 | cyp2ae1
Description
cytochrome P450, family 2, subfamily AE, polypeptide 1 [Source:ZFIN;Acc:ZDB-GENE-090312-107]
Coordinates
chr23:42687381-42687946:+
Coord C1 exon
chr23:42687381-42687578
Coord A exon
chr23:42687579-42687783
Coord C2 exon
chr23:42687784-42687946
Length
205 bp
Sequences
Splice sites
5' ss Seq
AGGGTAAGT
5' ss Score
10.45
3' ss Seq
GGTGAAATCTTGTTTGCCAGATT
3' ss Score
3.9
Exon sequences
Seq C1 exon
ATGTCATCAGTGTTCTCTCAGCTGATTGGCCAGTGGCTGGATGTTCAGGGATTCCTGATATTTCTCTGTGTGCTGCTGCTGGTGAAACACTTCCGAGATGTTTATTCAAAGAACATGCCCCCAGGACCCTTCCCTCTGCCTTTTGTTGGAAACCTCACAAACATCGGCTTTAGTGATCCACTGGGCTCCTTTCAGAGG
Seq A exon
GTAAGTCTCTTTATTCAGTTATGTGTGTGTGTGGTAAAATGATTTAGTGTGAATGTATAATGTGTAAAGACCTGTACACACCAAGCATGATCACTATAATGATATGGGGTGTAAACTATATCATTAGGTGAGTAAATAGTGAGTACATTTTCATTTTTGGGTGAACTTTCCCACCCATTTTTTTGGGTGAAATCTTGTTTGCCAG
Seq C2 exon
ATTGCAGAGAAGTATGGAGATGTGTGCACACTTTACCTGGGAACCAAGCCCTGTATACTGATGACTGGATATGATACTTTAAAAGAGGCTTTTGTGGAGCAGGCGGACATCTTTACGGACCGACCGTACTTTCCTATAGTGGACAAGCTTGGAAATGGGAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000013524:ENSDART00000035654:1
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion (1st CDS intron)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0006717=p450=PU(5.7=39.4)
A:
NA
C2:
PF0006717=p450=FE(11.9=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTCTCAGCTGATTGGCCAGTG
R:
GTCCGTAAAGATGTCCGCCTG
Band lengths:
304-509
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]