DreINT0054408 @ danRer10
Intron Retention
Gene
ENSDARG00000053530 | cyp4f3
Description
cytochrome P450, family 4, subfamily F, polypeptide 3 [Source:ZFIN;Acc:ZDB-GENE-070410-108]
Coordinates
chr12:48580947-48581765:-
Coord C1 exon
chr12:48581621-48581765
Coord A exon
chr12:48581001-48581620
Coord C2 exon
chr12:48580947-48581000
Length
620 bp
Sequences
Splice sites
5' ss Seq
CAGGTGGAC
5' ss Score
3.09
3' ss Seq
CACTCTTTCTGTTCTGGCAGCTT
3' ss Score
8.62
Exon sequences
Seq C1 exon
ATGGGTCATAATGAGGAGGGTCTGCAGCGTGTGGATGATCTGGTCTGTAAATACGGTCATTCCTGCTCCTGGTTTCTCGGCCCCTTCTATAACATGGTTCGTCTGTTCCACCCGGACTACATCAGATCCCTCCTGACCGCCTCAG
Seq A exon
GTGGACAACACACCTGTCAAACACACACACATCATACACACACACACACACACACACACACATCATACACATATAACTGGTGATTTCTCCTTGTAATGTGAAGCACACAAGATTACATAAACCACATGGCAAAACCATGATTTTCTACTTCAGTGTTGACAAAAACTCTGCCTTAAACACCAGCCATAACAATATATACAATAAAACTCCTACTTCTGTTCAAAAAACTAGCAAATATGCACAACTATATATATATAATCCAATATATATATATATATATATATATATATATATTTATATATATATATAGATCCAAAATCAAATAAATATATTAATTCAGTAATCAATAATGAATTAATATTGATAATCATGATAATACTAAATGTTTTCTGATCATCATATTAGATTGATCTCTGAAGGATCGCGTGTCTGCTGTAATGATGCTAAATTTCAGCTTTAAATCGCAGGAATAAATTACATTTCTGCACATATTCAACTAGAAAAAAATTATTTAAAATAGCACAAATATTTCACTTTTTTTGCTGTACTTTGCAACAATTAAATGCAGAATGTGAATTTATTTCTATAATAATAACTTTACACTCTTTCTGTTCTGGCAG
Seq C2 exon
CTTCCATTACTCTCAAAGACAGGATCTTTTATGGCTTTATGAAGCCCTGGTTGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000053530:ENSDART00000063442:2
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0006717=p450=FE(10.2=100)
A:
NA
C2:
PF0006717=p450=FE(3.8=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGTCATAATGAGGAGGGTCTGC
R:
TCATAAAGCCATAAAAGATCCTGTCT
Band lengths:
184-804
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]