DreINT0056534 @ danRer10
Intron Retention
Gene
ENSDARG00000014439 | dgkza
Description
diacylglycerol kinase, zeta a [Source:ZFIN;Acc:ZDB-GENE-111104-3]
Coordinates
chr7:38818210-38818486:+
Coord C1 exon
chr7:38818210-38818281
Coord A exon
chr7:38818282-38818390
Coord C2 exon
chr7:38818391-38818486
Length
109 bp
Sequences
Splice sites
5' ss Seq
GAGGTATGA
5' ss Score
8.55
3' ss Seq
CTTTCCCTTTTTTATTCTAGGAG
3' ss Score
11.66
Exon sequences
Seq C1 exon
TCCTCACTAAAGTCAAGCAAAAAGAAAAAACGGACATCTTTCAAACGTAAATCCAGCAAGAAAGGAGCAGAG
Seq A exon
GTATGAATACAGCAACAATTATTGTTATTATTTTTGTTTCTAAGAGGACAAAACAGTTAAAGAAGCAATATGTGTTTGTTTTATATACACTTTCCCTTTTTTATTCTAG
Seq C2 exon
GAGGCACGATGGAAGCCTTTTATAGTGAGACCCATTCCCTCCCAACTCATGAAGCCACTGCTAGTGTTTGTAAATCCAAAGAGTGGAGGAAACCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000014439:ENSDART00000093009:10
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.722 A=NA C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
PF0078119=DAGK_cat=PU(10.4=40.6)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCCTCACTAAAGTCAAGCAAAAAGA
R:
TCTTTGGATTTACAAACACTAGCAGT
Band lengths:
154-263
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]