DreINT0056589 @ danRer10
Intron Retention
Gene
ENSDARG00000076025 | dgkzb
Description
diacylglycerol kinase, zeta b [Source:ZFIN;Acc:ZDB-GENE-111104-4]
Coordinates
chr25:7736885-7737143:+
Coord C1 exon
chr25:7736885-7736934
Coord A exon
chr25:7736935-7737022
Coord C2 exon
chr25:7737023-7737143
Length
88 bp
Sequences
Splice sites
5' ss Seq
AAGGTATTC
5' ss Score
6.8
3' ss Seq
CTGCTTGATGTTTTCCTCAGTTG
3' ss Score
7.04
Exon sequences
Seq C1 exon
ATTCAACCAATTTGCTCTTTGATGCTGTGAAGTCTGGCAACTTGGAAAAG
Seq A exon
GTATTCATTTAATACTCAGTTCCAGTTGTATTATATATGAGGATTATTCGACCTCATGTTCTGTTTCTCTGCTTGATGTTTTCCTCAG
Seq C2 exon
TTGGAACAGATGCATGAAGAAGGAGCGGATCTCTCGGTCCAGGACCATTTAGGTTGCACCATCCTCCATCAGGCAGTCATTTTAGGTCACAAAGAGATTGTGAAGTTTATCACTGAAAACG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000076025:ENSDART00000155016:30
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.118 A=NA C2=0.220
Domain overlap (PFAM):
C1:
PF127962=Ank_2=FE(18.8=100)
A:
NA
C2:
PF127962=Ank_2=FE(47.1=100),PF127962=Ank_2=PU(0.1=0.0)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AACCAATTTGCTCTTTGATGCTGT
R:
CGTTTTCAGTGATAAACTTCACAATC
Band lengths:
167-255
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]