DreINT0059671 @ danRer10
Intron Retention
Gene
ENSDARG00000062083 | dph7
Description
diphthamide biosynthesis 7 [Source:ZFIN;Acc:ZDB-GENE-070410-57]
Coordinates
chr5:24575850-24576486:-
Coord C1 exon
chr5:24576421-24576486
Coord A exon
chr5:24576023-24576420
Coord C2 exon
chr5:24575850-24576022
Length
398 bp
Sequences
Splice sites
5' ss Seq
AAGGTAAGA
5' ss Score
10.57
3' ss Seq
ATAATGTCCCTGTTTTGCAGCTA
3' ss Score
8.91
Exon sequences
Seq C1 exon
GCACACTATGGGGGTTTGCAGCATACACAGTAACCCTCATCGAGAGCACATACTGGCCACAGGAAG
Seq A exon
GTAAGAAGTCAAATTAGTGTAAAAGACAGTGGCAATCACTTCACATGGGTATATAGCAGGCCATTATAGACAGACAGACTGATTTTGTTTATTATTCCTGCATTGTTATATTTGCTCCAACAGCTAAAAACACATTTCACATTTTTACAAAAGATTTTTAAAAAAAGCAGAGAAAAAATTTAGTATGCCAGTCAGAAGGAAGATGTTGATTTTACTGTCATTCCTCAACATCTGATTTAAGGTGCTTTTACATCTGCAAAACTTCATAGGCAAAAAAATATATATTTACTCCGTTTATACAGAGAATGCTTTCAAATCAGCCAGCTTTCTGTTGGTCAACATGGCAAATTATGGTTTGAAAACACCCTTAATAAATGCATAATGTCCCTGTTTTGCAG
Seq C2 exon
CTATGATGAGAATGTGTTGCTGTGGGATGGGAGAAACATGAAGCAGCCCCTGAGTGAGACTGCTGTGGGTGGAGGAGTGTGGAGACTCAAGTGGCACCCCAGTCAAGAGCACCTGCTGCTTGCTGCATGCATGCATAATGACTTCCAGATTCTATACTGCCAGAAAGCTTTGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000062083:ENSDART00000089339:7
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0040027=WD40=FE(55.0=100)
A:
NA
C2:
PF0040027=WD40=PD(22.5=15.3)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Human
(hg38)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CACACTATGGGGGTTTGCAGC
R:
CCAAAGCTTTCTGGCAGTATAGA
Band lengths:
238-636
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]