DreINT0062417 @ danRer10
Intron Retention
Gene
ENSDARG00000056765 | ela2l
Description
elastase 2 like [Source:ZFIN;Acc:ZDB-GENE-040511-1]
Coordinates
chr8:21338716-21339214:-
Coord C1 exon
chr8:21339117-21339214
Coord A exon
chr8:21338839-21339116
Coord C2 exon
chr8:21338716-21338838
Length
278 bp
Sequences
Splice sites
5' ss Seq
CAGGTTAGT
5' ss Score
8.02
3' ss Seq
TTTCTCTGGTGGCCCTGCAGCAG
3' ss Score
7.98
Exon sequences
Seq C1 exon
ATCTCCCTCCAGTACAAAAGTGGAAGCAACTGGTACCACACTTGCGGTGGAAGCCTTATTGACAAACAGTGGGTTTTGACTGCCGCTCACTGCATCAG
Seq A exon
GTTAGTTACAGAGAGAAGATCCTAAACACAGGCCTGGTTACAAATAAAAAACAACCATTTTAGTAACAGTTCATGATAACAGTATTAACACCATATATAAAAGTACCTTGTTTTTATGAAAAAAAAACATCTTAATTCCTTCATGTTTGTATTTTTCTAACTAAATCAGAAACTTTGTATTAGTCACACTTGAATGAATTGCTTCATTTGCGTTCTTTGATTACATTTTCTGACTAAATGTAAATGAAGTTATTTGTCTTTCTCTGGTGGCCCTGCAG
Seq C2 exon
CAGTAGCAGAACTTACAGAGTGTTTTTGGGAAAACATAGCCTGAGCCAGGAGGAGAACGGCTCTGTCGCTATTGGTGCTGGAAAGATCATTGTCCACGAGGCTTGGAACTCCTTCACAATCCG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000056765:ENSDART00000079293:3
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0008921=Trypsin=FE(13.8=100)
A:
NA
C2:
PF0008921=Trypsin=FE(17.7=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATCTCCCTCCAGTACAAAAGTG
R:
GGATTGTGAAGGAGTTCCAAGC
Band lengths:
220-498
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]