DreINT0066872 @ danRer10
Intron Retention
Gene
ENSDARG00000089357 | fam73b
Description
family with sequence similarity 73, member B [Source:ZFIN;Acc:ZDB-GENE-050320-135]
Coordinates
chr21:3705872-3707159:+
Coord C1 exon
chr21:3705872-3705989
Coord A exon
chr21:3705990-3707052
Coord C2 exon
chr21:3707053-3707159
Length
1063 bp
Sequences
Splice sites
5' ss Seq
TGGGTAAAA
5' ss Score
3.28
3' ss Seq
TGCGTAATTTGTCCACACAGAAA
3' ss Score
7.2
Exon sequences
Seq C1 exon
CCCGAAGTTCGTAATCATCAGTTTGCAGAGAAGCTGGAGACTCTGCTGCACAGAGCTTATCATCTGCAGGAGGACTTCGGCAGCACAATCCCACCTGACAGTCTGCTTGCAGATCTGG
Seq A exon
GTAAAATATACACAAATCCATGCTAACCAAACACATTTCAGGTTCTTAAAGGAACACTCCATTTTTTTTTTTTTTTTTGGAAATTGGCTTTTTTTCTGCCTCTCCTAGAGGTAAATAGTTGAGTATCACCACCACATAATACTTAATACACACATGCATGGAAACAAAACTATACAAAACAAGTTTGTTGCTAGCGTATATAAATAAATATATCAAATGTATCATATGCATGTATAATTTATTCTAAATATAATTTTTTTTTTCCACAAATATATGCATGTATTTATGTATAAATATTAAATAAACAACATACACACACTTAAACTGTCAACACAAACTTTTATTTTGTATGCGATCAATCATGATATAGTTTTACTCAGCACTAGTTATTATGCATGTTTTTTGGGGGGGGTTTGCATTTAAAAAATATTCATTGCTTGTTCATGTTAGCAAATACATAAACTAACATAAACTATGAATGTAACAGTTGTTTTGTCTAATTTTAAATCATCCTAAGTTATCCTGAAAATACCTTTTTATTTTATAATTTATATTTATATATATATATATATATATATATATATGTCTTTAGACATTTTGTTTATTATTTATTTTTATTTAAATTTTAATATTTTTAATATATATTTTAAATTTTTTTATTTAATTTTTATATATTTATTTTTATTAGTTTTTCATTAAATTTTTATTTATTTTTACTTATTTATATTTATTTATTTTTATTTATTCATTTTAAATGTATTTTTATGTATTTATTTTTATTTATTATTTGTATTTATTTATTAATTTTTTAATTAATTACTTTTTATTTTTTAAATGTATTTATTCGTTTTTATTTCTTTATTTAGTTTTTATTTATTTATGTATTTATTTAATATTTTTATTTATTTATTTATTTAGTTAGTTTTTAGTTAATTATTTTTAATAATTTATTTATTGTTATTATTTTATTTTTGTTTACTTATTTTAATTTTTTTTTATTTATTTAATTTAATTTTATTTAGTTTATGTTTTTTATATGACCTTGCGTAATTTGTCCACACAG
Seq C2 exon
AAAGTGAAGGCACGCTGATTTTGCCCACTCTGGGAAGTTCACACCCCATCCAGGATGACGATGCCACGACGGTCACATCGGACGACTCGTTTTTCTCTGCTGCTGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000089357:ENSDART00000122699:5
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.175 A=NA C2=0.111
Domain overlap (PFAM):
C1:
PF102654=DUF2217=FE(8.5=100)
A:
NA
C2:
PF102654=DUF2217=FE(7.7=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCCGAAGTTCGTAATCATCAGT
R:
AGCAGCAGAGAAAAACGAGTCG
Band lengths:
222-1285
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]