DreINT0078065 @ danRer10
Intron Retention
Gene
ENSDARG00000039452 | hk1
Description
hexokinase 1 [Source:ZFIN;Acc:ZDB-GENE-040426-2848]
Coordinates
chr13:22855473-22857385:-
Coord C1 exon
chr13:22857286-22857385
Coord A exon
chr13:22855657-22857285
Coord C2 exon
chr13:22855473-22855656
Length
1629 bp
Sequences
Splice sites
5' ss Seq
TAGGTAAAT
5' ss Score
6.62
3' ss Seq
ATGTTGCTGTGATCCCTCAGGTA
3' ss Score
9.45
Exon sequences
Seq C1 exon
GATTACGAAGCAGATATAATGGCTGTTGTCAATGACACTGTTGGAACTATGATGACCTGCGGATTTGATGACCAGCGATGTGAAGTCGGCATTATCATAG
Seq A exon
GTAAATAATAATAATAATATTATATCCTTGTTCAAATTTTATCTATACTTGCATCTACACTCACCGGCCACTTTATTAGGTACACCTTACTTGCAGGTTGGCTCCCGTTTTGCCTTTAGAACTGCCTTAATCCTTCATGGAATAGATTCAACAAGGTACTGGAAATATTCCTCAGAGATTTTGGTTCATATTGACAGTTCCGCCACATCCCTAAGTTGCTCTATTGGATTGAGATTTTGGTACCGTGGAGGCCATTTGAATACAGTGAACTCATTGTCATGTTAAAAAAAAAAACAGTCTGAGATGATGCATGCTTTATGACATGCCGCATCATCTTGCTGGAAGTAGCCATCAGAAGATGGGTACACTGTGTTCAAAAGGGATAAACATTGTCAGCAACAATACTCAGCTAGGCTGTGGTGTTGACAAACTGCCGATCACTAGATATTTTCTCTTTTTCTGACCATTCTCTATAACCTCTAAAGACGGCTGTGCGTAAAAATCCTAGTAGATCAGCAGTTTCTGAAATACTCAGACCAGCCTGTCTGGCACCAACAACCATGCCACGTTTAAAGTCACTTAAATCATTTTTCTTCCCCAATCTGATGCTGGGTTTGAACAACAGCAGATCATCTTGACCATGTCCACATGCCTAAATGTATTGAGTTGCTGCCATCTGATTGGCTGATTAGAAACTTGCGTTAATGAGCAGTTGGACAGGTGTACATAATTAAGTGGCTGGTGAGTGTGTATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATAGACAAGGCAAACTTTATTTATAGAGCACAATTTTACACAACTGTAGTTGATCCAAAGTGCTTTACAATAATAATATATAGTATATAAGCTTTAATTACTATACTTGATTAGTCCTCTGCTTGTACCTCACTCAAGTGCACAAACACATTTTGAAACAGTGAGCATACACCCAGACAGTTATTGCTTCCAGCACAACAGAGAGCTCAAGAGCACCTCACCAAAGTATCAAAGATGGAGATTCATTCTCTGCCCCAGCTTCAATTCCCTATGTCTGTTGTAAAATAGCTTTTTTCAATGTGTAGGTGGGCAAATTTTCAAAACCTACACCATAATTGGAGTTACTGAGTTACAAACATAAAAACAAATTCATAACTGCCTAAAACGAGTGGCAATAAATCCAGTCTCACTTCCTGCTCCAAGTAATAACATCAATCTTGATTGGCTGCCAGTGAACAAAGTCTCAATTTGAGAGGCCTAAAAGAGTTTTTTTGTGTTGCTGGCATTTAGGGAAGATAGTGTTTTCTGTGTCGTAAAATCAAATTCACTTTCCAAGGAGTCAATTTCAAATTTATCCCTTTATAATTTAAATAGTCCTTTAAAATAGCACTATAGGGTTAAATTAGTTCAAGTCTAGGGTTTTAAAGTTGTATTTAGCTTTTTTTTGTTTTGTTTTCAGTAATATTGAAGAATAACATTGTTATTCAATGTTCAAATTAAGAGGGAAAACTAGGAATATTTCTAATATTCTAAATATACTCAATCAAAGCTTTTGGATTTCTGAACATCTGCGATGACATATGTTGCTGTGATCCCTCAG
Seq C2 exon
GTACCGGCACTAATGCATGCTACATGGAGGAGCTCAGACACATGGACATGGTGGAGGGAGATGAAGGCAGGATGTGTATTAATACTGAATGGGGGGCGTTTGGAGATGACGGCACATTGGAGGACATCCGGACTGAGTTTGACAGGGAGATTGACCGAGGATCCCTCAACCCTGGCAAGCAACT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000039452:ENSDART00000057638:6
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.016
Domain overlap (PFAM):
C1:
PF0034916=Hexokinase_1=PD(11.2=67.6),PF0372711=Hexokinase_2=PU(3.3=23.5)
A:
NA
C2:
PF0372711=Hexokinase_2=FE(25.4=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACGAAGCAGATATAATGGCTGTTGT
R:
AGTTGCTTGCCAGGGTTGAG
Band lengths:
280-1909
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]