DreINT0078093 @ danRer10
Intron Retention
Gene
ENSDARG00000038703 | hkdc1
Description
hexokinase domain containing 1 [Source:ZFIN;Acc:ZDB-GENE-030131-9801]
Coordinates
chr13:22887710-22889622:-
Coord C1 exon
chr13:22889467-22889622
Coord A exon
chr13:22887944-22889466
Coord C2 exon
chr13:22887710-22887943
Length
1523 bp
Sequences
Splice sites
5' ss Seq
GAGGTAACA
5' ss Score
8.1
3' ss Seq
GGCTTTTCCCATTTGCCTAGTGA
3' ss Score
5.09
Exon sequences
Seq C1 exon
GTTTGAAAAAATGACCAGTGGCATGTATTTAGGAGAGATTGTAAGGCAGATCCTTATTGATCTCACAAAGCGTGGTTTTCTGTTTCGCGGTCGCATCACAGAGAGACTGAAGACCAAGGGAATCTTTGAAACCAAGTTTCTTTCACAGATTGAGAG
Seq A exon
GTAACACAACACAATACATTCCTCATATAACTGCCCTCACTGATATTTTGGAATGTACCCTAAATAAATGAGTAAATTAACAGGACGCTTTAAGTAAAGAACCACTTTTAAATGCAGTACCTAAGTCCTAATAAATCAAAGTAACTAATGCATGTACATGGGAAAATAATGAGTGCACATTTAATAATATATATTCAATTCTGTCAAATGAATATTTTAGTAACAGGCATTTAAAGTCACCTTCCTATAATATTACGATTGTATTGACAACTATATTTTCAGACTGTTTAATTTTATCAAATATCATAACAAAGTGAAGAGCTGGGTTAAATAAAAGACACATCGGCTATAGCTTGTCAAACTCTGACTAGTTATCTCAGGGATTTGAGATAACAAAAAGGCAGAGGTTATTTATAGCCATTTTCTGGACTCTGAATAGCAGTAGAGTCAGGGTCGACTCCAAAATAGGAATCAACCCTTGTTGTTGGCTCCATTGTGTCTAAAATCACTCACTTGTTCACTGATTCTCTACCCCTACACACTTGAGTGCACTATATTAGTGTAGAGTGAGCAAAATTAAGCAAGGAATTCGTACATAGTGTCTGGTGTCAGAGGAAGGGTTCTTACACCTTTTCCAAAGTCAGATTTAAGGACTTTTCGAAGGACTTTAGCTCTTTGACATCAGCTTAGGCAGTACCCAAACCTTGTAAGACAACAGGAGGGTTATACTTCTTACTTTCTATTCTCTATATCAGTGGTTCTCAATCATAGTTCTAGCTTGACCCAGTTTTCAGAATATTTTGCATGTCTCTCTTATTTTACCTACCTGATTCGAATATGAAGCTCATTAGAGGAGAGCTCCATGCATGAACTGTTCTTCATACACAGTGATCAATGCTCAATACTCCCTGAGCAAAGGGAATCTTCACTTTGGAGTTTTTATTGGCAGATTTATGCAGCACCATAGTCTACAGCGTCTTCACACAAACAACACTTGTGAAGAGATATAATGCATCTCTGAAAATGAATACAAAGATCTGTCATGAAACTCTATGCATACAGGTTGATTGGTCACACCTAATCTGCTCCTACATCATTAACCACATGGCATAGGTGCCACCCCAGCTCCACCTGTGTTTATATCTGCTATAGGGGTTAGATATATAACCATACATATATTAGAGATATATTATGCTTGCAGCAGTAGTGTGTTATGTTCTCAGACTGAATGTCTGTGAGTATACTGTACTAGAAGTTCCAAGTGTCGGTACTTGCTCTGCCATAATAATCAAAGCAGCAGCAGCGTAGCAGTTCTATGCCGCCAAGACCAAACACATCAACATTGCCATACACTGTACATATTCATTACCATACTAAATCTCTCCATCAGTTGTCATGACAGAATACAGTTTAAATTGAGATATTGCACACTTCTGTGCCATTTGAAGAAAACAATGAACTGGAAACATTAATTAATAATAGTTTATTCATGACTTGCGCTTCGGCTTTTCCCATTTGCCTAG
Seq C2 exon
TGACCGTTTGGCACTACTGCAAGTGAGGTGCATATTGCAGAGTCTGGGTCTGGACAGCACATGCGATGACAGTATTATTGTGAAGGAGGTTTGTGGAGCTGTTTCTCGTCGAGCAGCTCAGCTCTGTGGAGCAGGAATGGCTGCAATTGTGGATAAAATCAGAGAGAACCGTGGATTGGATCGTCTGGATATCACTGTTGGAGTGGATGGCACTCTTTACAAGTTACATCCACA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000038703:ENSDART00000056523:16
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0372711=Hexokinase_2=FE(21.7=100)
A:
NA
C2:
PF0372711=Hexokinase_2=FE(32.5=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGACCAGTGGCATGTATTTAGGA
R:
GTGCCATCCACTCCAACAGT
Band lengths:
358-1881
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]