DreINT0086514 @ danRer10
Intron Retention
Gene
ENSDARG00000103394 | kif5bb
Description
kinesin family member 5B, b [Source:ZFIN;Acc:ZDB-GENE-070629-4]
Coordinates
chr12:41052733-41052984:+
Coord C1 exon
chr12:41052733-41052834
Coord A exon
chr12:41052835-41052923
Coord C2 exon
chr12:41052924-41052984
Length
89 bp
Sequences
Splice sites
5' ss Seq
CACGTACGA
5' ss Score
5.74
3' ss Seq
ATGATTGAATCTGTTTTCAGGGT
3' ss Score
8.33
Exon sequences
Seq C1 exon
TCAAAACCAGAAGATCATTTTGGAGCAGGAGCGCTTGAAGGTGGAGCATGAAAAACTCAAATCCACTGACCAGGAGAAGAGCCGCAAACTGCACGAGCTCAC
Seq A exon
GTACGACACACACCGAATCAATGAAGATCTGAGTAGTTTGCAGGAGAAGAGTTTAGTAATGGTGTGTTAATGATTGAATCTGTTTTCAG
Seq C2 exon
GGTCATGCAGGACCGAAGGGAACAGGCCAGACAGGATCTCAAAGGCCTGGAGGAGACAGTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000103394:ENSDART00000170976:20
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.543 A=NA C2=0.905
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AACCAGAAGATCATTTTGGAGCA
R:
CACTGTCTCCTCCAGGCCTTT
Band lengths:
159-248
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]