DreINT0086521 @ danRer10
Intron Retention
Gene
ENSDARG00000103394 | kif5bb
Description
kinesin family member 5B, b [Source:ZFIN;Acc:ZDB-GENE-070629-4]
Coordinates
chr12:41035288-41035553:+
Coord C1 exon
chr12:41035288-41035336
Coord A exon
chr12:41035337-41035497
Coord C2 exon
chr12:41035498-41035553
Length
161 bp
Sequences
Splice sites
5' ss Seq
ATGGTATGT
5' ss Score
8.35
3' ss Seq
ATTTTTTATTCCACTTTTAGTAT
3' ss Score
7.97
Exon sequences
Seq C1 exon
GTGTCCTATTTCGAGATCTACCTGGACAAGATCAGGGATTTGCTGGATG
Seq A exon
GTATGTCCTACTAAATATGTTTAGTTTTGGATTTATTTGTGTTGATTCTATATTAAAGTCTTCTGTCGCATTAGAATAAGCTAATTAATAGTATTTTAATTAATTTTTTAACAGAATGTCCGTAAATATGCAGATTCTTCTATTTTTTATTCCACTTTTAG
Seq C2 exon
TATCTAAGACCAACCTGTCTGTACACGAAGACAAGAACAGAGTCCCTTATGTAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000103394:ENSDART00000170976:5
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0022518=Kinesin=FE(5.1=100)
A:
NA
C2:
PF0022518=Kinesin=FE(5.8=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTGTCCTATTTCGAGATCTACCTGG
R:
TAAGGGACTCTGTTCTTGTCTTCG
Band lengths:
98-259
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]