DreINT0099043 @ danRer10
Intron Retention
Gene
ENSDARG00000070331 | muc5.1
Description
mucin 5.1, oligomeric mucus/gel-forming [Source:ZFIN;Acc:ZDB-GENE-120822-1]
Coordinates
chr25:8294855-8295292:+
Coord C1 exon
chr25:8294855-8294952
Coord A exon
chr25:8294953-8295140
Coord C2 exon
chr25:8295141-8295292
Length
188 bp
Sequences
Splice sites
5' ss Seq
TGCGTAAGT
5' ss Score
9.73
3' ss Seq
TTATTTTGTTTCGGCTGTAGGTG
3' ss Score
9.51
Exon sequences
Seq C1 exon
ACTGCGTGGCTCCAATGACATTCTTTAAATGCTCACATCCTGGAGAGAAAGGCACAGAATGTCAGAGGACATGCGACAAACAAGACCCAAACAATTGC
Seq A exon
GTAAGTTAAAACAGTTGAATACAGTACATAACAATAAGATTACAGGCATTACTCAGGCATATAAAATATATACAGTCAAGTCTGAAATTATTCATACCAAAGAGAGAGAGAGCATGTTATGAAATAGTTTGTTCTACTGTAGACATTTGCTAAGAAAAGTTGTAAAACTTATTTTGTTTCGGCTGTAG
Seq C2 exon
GTGAGCACAGGGTGTGTATCAGGGTGTATGTGTCCTGAGGGCCTTTTGGCTGATGGAAAAGGTGGATGTGTGATGAGGGAAAATTGTCCATGCACCCATAACGGTGTGACAAGTTCACCTGGGGATCAAGTTCAACAGGACTGTAACACCTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000070331:ENSDART00000153708:20
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0182612=TIL=PU(43.4=69.7)
A:
NA
C2:
PF0182612=TIL=PD(52.8=54.9),PF0009313=VWC=PU(32.8=39.2),PF053758=Pacifastin_I=PU(41.2=27.5)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCGTGGCTCCAATGACATTCT
R:
CAGGTGTTACAGTCCTGTTGAACT
Band lengths:
247-435
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]