DreINT0099802 @ danRer10
Intron Retention
Gene
ENSDARG00000100972 | myh11b
Description
myosin, heavy chain 11b, smooth muscle [Source:ZFIN;Acc:ZDB-GENE-101124-2]
Coordinates
chr3:36555152-36555928:-
Coord C1 exon
chr3:36555729-36555928
Coord A exon
chr3:36555274-36555728
Coord C2 exon
chr3:36555152-36555273
Length
455 bp
Sequences
Splice sites
5' ss Seq
AGGGTATGC
5' ss Score
7.47
3' ss Seq
ATCCCCATTTCTATCCCCAGGCT
3' ss Score
8.97
Exon sequences
Seq C1 exon
TGGACCGTGTGGTGGGTTTGGAAACCATGGCTAAGATGGCCAAGTCTGACAGCTCAGCTCCTGCTGCTTCCAAGTCCAAGAAAGGCATGTTCCGCACCGTCGGGCAGCTGTACAAAGAGTCCCTCGGCAAACTCATGACCACTCTTCATAACACTCAACCAAACTTTGTGCGCTGTATCATTCCCAACCACGAAAAAAGG
Seq A exon
GTATGCCACAGCAATCATCCTTTAATGTGGTTTAAACCTACTGGTACAACTTGTGTATATTGGCCGAAAAAGCTCAAACACACTGCAAGGAATACACATGCATGGTTCAAATCTACTGTACAACTTTGCCTTCTTTAAAGGGCAGATATGTACATTGACCGAAATAGCTCAAACACACTGCAAGATCTGTTTGACAACACATGCACAAACAGAAACATGTGGCAAATAACGCATGAAACAAAAAGTAAAAAAATCTGTCTGCTTCCTATTTAGTCAATGGCATTGTTGGAGAGCTAAAAGTTTTTTTGTTCATTCGAACGCCCTGGTTGTTCCTTGTTGTTTGTGTATAGAAGCGGCCAGATTCATCACTTTTTGGAGACTCCTGACACATTTGCTCAGCACTGTTGGAGTGTGATGTTGTATTTGAAAAAAAAAATCCCCATTTCTATCCCCAG
Seq C2 exon
GCTGGCAAGATAGATGCCCATCTTGTTCTAGACCAGCTGAGCTGTAATGGTGTACTAGAAGGGATCCGCATTTGTCGCCAGGGCTTCCCTAATCGTATCGTCTTTCATGAGTTTCGCCAGCG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000100972:ENSDART00000166896:14
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.060 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0006316=Myosin_head=FE(9.8=100)
A:
NA
C2:
PF0006316=Myosin_head=FE(6.0=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Rat
(rn6)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCGTGTGGTGGGTTTGGAAA
R:
CGCTGGCGAAACTCATGAAAG
Band lengths:
318-773
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]