DreINT0099811 @ danRer10
Intron Retention
Gene
ENSDARG00000100972 | myh11b
Description
myosin, heavy chain 11b, smooth muscle [Source:ZFIN;Acc:ZDB-GENE-101124-2]
Coordinates
chr3:36548896-36549276:-
Coord C1 exon
chr3:36549153-36549276
Coord A exon
chr3:36549068-36549152
Coord C2 exon
chr3:36548896-36549067
Length
85 bp
Sequences
Splice sites
5' ss Seq
AAGGTGTTT
5' ss Score
2.06
3' ss Seq
ATTACTGTGCGCCTGTGCAGTTC
3' ss Score
6.62
Exon sequences
Seq C1 exon
GAGAAGCAACAGCTGGAGGAGCGGTTAGCTGACTTTAGCTCCAACCTTGCTGAGGAGGAAGAAAAATCTAAAAACCTGACTAAACTAAAGGCTAAACATGAATCTATGATTTCAGATCTTGAAG
Seq A exon
GTGTTTTAACGTTTCTTTTAATCATCTACATTATTAAATAATATTGTCAATACCTTAAAACAATCATTACTGTGCGCCTGTGCAG
Seq C2 exon
TTCGAATGAAGAAGGAAGAGAAGAGTCGTCAGGACGTAGAGAAAGCAAAACGTAAACTGGAGACAGAGCATAGTGATTTACAGGAACAAATGAAGAACCTGCAGACCCTGATAGCAGAGCTTAAGACACAGCAAGCCAGAAGTGAAATGGAAATTCAAGAACTTCAAGCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000100972:ENSDART00000166896:22
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=1.000 A=NA C2=1.000
Domain overlap (PFAM):
C1:
PF0157614=Myosin_tail_1=FE(15.2=100),PF045827=Reo_sigmaC=PU(25.5=85.7)
A:
NA
C2:
PF0157614=Myosin_tail_1=FE(21.2=100),PF045827=Reo_sigmaC=FE(40.4=100),PF0157614=Myosin_tail_1=PU(2.4=34.5)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GAGAAGCAACAGCTGGAGGAG
R:
ACGTTTTGCTTTCTCTACGTCCT
Band lengths:
177-262
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]