DreINT0099814 @ danRer10
Intron Retention
Gene
ENSDARG00000100972 | myh11b
Description
myosin, heavy chain 11b, smooth muscle [Source:ZFIN;Acc:ZDB-GENE-101124-2]
Coordinates
chr3:36548048-36548515:-
Coord C1 exon
chr3:36548371-36548515
Coord A exon
chr3:36548255-36548370
Coord C2 exon
chr3:36548048-36548254
Length
116 bp
Sequences
Splice sites
5' ss Seq
CGGGTGAGA
5' ss Score
7.62
3' ss Seq
GGTCTAATCTTGAAAAACAGGCC
3' ss Score
2.76
Exon sequences
Seq C1 exon
GGCTAAGAGGGAGCAGGAGGTGGCTATGTTGAAGAAGTTGATAGAAGATGAAGGCCGAAGCCATGAGGCTCAAGTGCATGAGCTGAAACAAAAACATGCTCAGGCTGTAGATGAACTTAGTCAGCAGCTAGACCAATCTAAACGG
Seq A exon
GTGAGAAAACTGCTTAACACTGGCAAAAAAAAAGTTCAAAATGTTTAAGTTCACTTTGGATATAAGCATCTGCAAAATCATTAAATATAAGCAATGGGTCTAATCTTGAAAAACAG
Seq C2 exon
GCCAAAGCCACTCTGGAGAAGGCTAAACAAGCCCTTGAGAAGGAGGTTGGAGACCTAAATGGAAACCTTCGCTCACTGGGTAATGCCAAGCAGGATTTGGAGCAGAAGAAGAAGAAGGTAGAGACCCAGCTTGCTGACCTTCAGACTCGTTTCAATGAGAGCGAGAGAAAAAGGGAGGAGCTTGGAGATGCAGTGTCCAAACTTAAT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000100972:ENSDART00000166896:25
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.898 A=NA C2=0.957
Domain overlap (PFAM):
C1:
PF0157614=Myosin_tail_1=FE(5.6=100)
A:
NA
C2:
PF0157614=Myosin_tail_1=FE(8.0=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTAAGAGGGAGCAGGAGGTGG
R:
TCTGCTCCAAATCCTGCTTGG
Band lengths:
249-365
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]