MmuINT0103273 @ mm9
Intron Retention
Gene
ENSMUSG00000018830 | Myh11
Description
myosin, heavy polypeptide 11, smooth muscle [Source:MGI Symbol;Acc:MGI:102643]
Coordinates
chr16:14209604-14211463:-
Coord C1 exon
chr16:14211319-14211463
Coord A exon
chr16:14209811-14211318
Coord C2 exon
chr16:14209604-14209810
Length
1508 bp
Sequences
Splice sites
5' ss Seq
AGGGTACGT
5' ss Score
9.15
3' ss Seq
CTCTCTACTTCCCTTGACAGGCC
3' ss Score
10.37
Exon sequences
Seq C1 exon
AGCCAAGAGGGAACAGGAGGTGACAGTGCTGAAGAAGGCCCTGGATGAGGAGACGCGGTCCCATGAGGCCCAGGTCCAGGAGATGAGGCAGAAGCACACACAGGCAGTGGAGGAACTCACAGAGCAGCTGGAGCAGTTCAAAAGG
Seq A exon
GTACGTGCTCAGGAGATACCACAGAAACCACGTCATGATGGCAGACTTCACCCACTCTTGTCTAGAGCTTTGGAATTTGAAATGCTCCCAAAAAACCCTAGAACTTTCTGTGTGTTACTAGTAAAAATTCCTACCAGTGCATTCATGAGATGGTCACCGTCAGAATTCAAGTATACCAAGTTAATTGATAAAGTGATCTTCAGGCCATGCAGACGGTGTCTATGAAAAGCAGGCAAGTCATGTTCAGTCTGAGTCCTGTGGCTCCATTCCCCACGCTGCTTCTTTATACTCAAATACAAATATCCTACAACTCAGAGAGCTGTGAGATCCATGGTTCTCAGATTTACAGAAATTGTGTACCCTAGATATATATCATGTGTGTGGGAGAGCAGGGGGATGATCTGGAAGCAGGTGGTTTGCCTGGGTTCCAGGAGCCACATGGTTTTCTCTTCTTCTATTCTCCTCACTAGCTCAAGTCCATCTCTTTTTCCTGTTTTTATCCACTCTTTTTACTTAGGTGACTCATACACACACACACACACACACACACACACACACACACACATATATATGAATGAGCTGGTTAGGTAAAATAACTTGATCACAGAAATATCAGGGCAAATTAGACAATGAATGCAAAAAAAAAAAATGAGAAAGTGATGGTAACCAGACCAAAGGGAGATGGGCTGCGGCATGAACAGCAGCCACCACCTAGATCTGACTGGTTTCTGCCTTGGGGGAAGGTATAACTTCTGTCTCCAGGTCTTTAGCTTGTTCTTTGAAAGTCAGAATTATTGAAACATGCAAAATTGTTGAAATATGTAAAATTATTGAAATATGTAAACAGACCACATGCACACATGCGCATGCGCACGTGCACACGCACGCGCGGGCGCGCACACACACACTTTTCTTATTTTTAGACAGAGTCTCATTCTATAGCCTAGGTTGTCTTAGAACTCATGGTAATCCCCCAGCTTCAGCTGGAATGCTGGGAATACAGACGTGAGCCACCATGGCCTGCCTCCCTTTTTTTCTCTTTCCCCTTGTTTCTTTCTCTCTCTCACCCTCTTCCCTCCTCTCTCTTTCTTCTTTGAGACATGATTTCACTAGGTAACTCGAGCCTCACATTCAGGATTCTACTACTGAGTGCTGGGATTAGAGGTCTATGCCACCAAACCTGGCTAAATTCACCAACTTTAAGTGTACCTTTCAATGCATTTTGACAAATGTGGCCATCTGAGCAGAGTGCCACAGTCACAACATGAAGTTGTTTTTAAAAAATAAATCTGGGAATTCAGATTTTTCCTGGAAATGTCCTAAAATTTGGTAACCTATACAGAACAGGCCTCAGGGACATGGATATGTTTTGATTTGATTCCTATAGTTCAGAAACTAGAATTTCAAGAGTCCTGCATATGTTCTATGCACCCCTCCTCCCTTCCCTTGGACTGGGAGTGGGGCAGGGAGAGGAGACAAGCCCACCCCCTCTCTACTTCCCTTGACAG
Seq C2 exon
GCCAAGGCAAACCTGGACAAAAGCAAGCAGACACTGGAGAAGGAGAACGCGGACCTGGCTGGGGAGCTGCGTGTCCTGGGCCAGGCGAAGCAGGAGGTGGAACACAAGAAGAAGAAGCTGGAGGTGCAGCTGCAGGATCTGCAGTCCAAGTGCAGTGATGGGGAGCGTGCCCGGGCTGAGCTCAGTGACAAGGTCCACAAGCTACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000018830-Myh11:NM_013607:27
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.837 A=NA C2=0.870
Domain overlap (PFAM):
C1:
PF0157614=Myosin_tail_1=FE(5.6=100),PF045827=Reo_sigmaC=PU(13.9=40.8)
A:
NA
C2:
PF0157614=Myosin_tail_1=FE(7.9=100),PF045827=Reo_sigmaC=FE(47.2=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Cow
(bosTau6)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCAAGAGGGAACAGGAGGTGA
R:
TGTGGACCTTGTCACTGAGCT
Band lengths:
342-1850
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: