DreINT0099820 @ danRer10
Intron Retention
Gene
ENSDARG00000100972 | myh11b
Description
myosin, heavy chain 11b, smooth muscle [Source:ZFIN;Acc:ZDB-GENE-101124-2]
Coordinates
chr3:36545768-36546300:-
Coord C1 exon
chr3:36546094-36546300
Coord A exon
chr3:36545981-36546093
Coord C2 exon
chr3:36545768-36545980
Length
113 bp
Sequences
Splice sites
5' ss Seq
AATGTGAGT
5' ss Score
7.8
3' ss Seq
AAACATAACATTTATAAAAGGCC
3' ss Score
0.33
Exon sequences
Seq C1 exon
ATGCTTGCGGATGAGAAGACCCTCTCCAGCAAATATTCTCAGGAGCGTGACTGCGCAGAGGCAGAGGCACGGGAGAAGGAGACCAAATGTTTGGCCCTCACTCGAGCGCTGGAGGAATGCCAGGGTTCATTACGTGAGTTAGAGAAACTCAACAAGACACTTCGCACTGACATGGAAGATCTAATCAGTTCAAAAGACAACAAAAAT
Seq A exon
GTGAGTTGCATTTTAAAGCCGAATGAAAATTGGAGAGAAGTTAATTGTAAATGTGAAGGTGTACAGTAGTCATCTGAATGTAAACAATAATAAAAACATAACATTTATAAAAG
Seq C2 exon
GCCCATGAGCTGGAGAAGACCAAACGTGCACTGGAGGCTCAAGTGGAAGAAATGACAATTCAGATGGAGGAGCTTGAAGATGAATTACAGGCTGCGGAGGATGCTAAGCTCCATTTGGAAGTAAATATGCAGGCCCTCAAAGTCCAAATACAAAGAGACATCCAAGGCAGAGAGGAGCAAAGTGAAGAGAAGAGGAAACAACTCCTCAAGCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000100972:ENSDART00000166896:30
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.580 A=NA C2=0.831
Domain overlap (PFAM):
C1:
PF0157614=Myosin_tail_1=FE(8.0=100)
A:
NA
C2:
PF0157614=Myosin_tail_1=FE(8.2=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATGCTTGCGGATGAGAAGACC
R:
TCCAGTGCACGTTTGGTCTTC
Band lengths:
242-355
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]