DreINT0099836 @ danRer10
Intron Retention
Gene
ENSDARG00000073732 | myh14
Description
myosin, heavy chain 14, non-muscle [Source:ZFIN;Acc:ZDB-GENE-100921-1]
Coordinates
chr16:16977199-16979466:+
Coord C1 exon
chr16:16977199-16977342
Coord A exon
chr16:16977343-16979251
Coord C2 exon
chr16:16979252-16979466
Length
1909 bp
Sequences
Splice sites
5' ss Seq
CATGTAAGA
5' ss Score
5.58
3' ss Seq
CATACTGTCTTTCTTTTCAGCCA
3' ss Score
10.12
Exon sequences
Seq C1 exon
AGGAGCTTTTGCTGGGAGGTGCAGATCAATATCGTTTCCTCTGTGGAGGTTCACTTCCTGTTCCGGGTCAAAGTGATTCAGAAAACTTCACTCAGACAATGGACTCGATGACCATCATGGGCTTCACCCAGGAAGAATCTACAT
Seq A exon
GTAAGAGAGAAGAGGGGTCTCTAGAAGGGGTCAGAAATTATCCTCAAGTAACTGTACATATTCAGAATGCAAGTTTTGCTCTATTAAAAGCATCTGGGCAAAAATAAATTTGAGTTAAATTAAAAAGTTCAGCTTTTAGTTGTACTTGTAAAAGTAAAAGTACAGTAGCAAGAACAAAAATGAGATATGAAATTATTCATACATCATATCGACTCATCCACTAATGCATTAAATGAAAAGTATTATAGTAAATTATTATTAAAAGTAAAATGATAACTCATCACACATTTTCATCACTTGATGATATTTGCACTCATACAATATTGTTTATTCTGCCAATATACTAAAAAGACAGCTAATGCATATGCAGCTAAAGAGAAAAACAAAACCTTAAATTGTTTTTTTTTTTGGATGCTCTGGGTTTACTAGGTATATTTTTGAATAAAACAGATATATTTATTAAAATAATATTTTAGTGACCAAACATATTAAGAAATTAAAAGACAATAAAATTAAAATTCAAGCAAAATATTGCAAAACATATTACAACCTACAAAATTTCAACAATTTTTTTCTATTTTTTTTTGCTTCTCTTGATTTTTTCTTATTTTTTTTTAAATGTATTTTATATTTTATATCTTCTATAACATGTTAATTTGGGTTTAGTAGTTTTTGGATTATTATTGTAAGCTATTTTGTTGGATAGACTCCAGATTTGGCTTCAGTAGTGTTTAATCTAATGTATATAAACAAATATAATACTGTATAGATTCCTATTAACATATCATTTTAAAAGATAGATTTGTTAGGGGTGTACTTATACTGTTGAAATCAAAATTATTAGCCCCCCTAAATTATTAGCTCCTCTGTTTATTTTTTTCCTAATTTCCTTTTTTGTTTAACGGAGAGAATATGTTTTTCCAACACATTTCTAAACATAATAGTTTTAATTACTCAATTCTAATAACTGATTTATTTTATCTTTGCCATGATGACAGTAAATAATATTTGACTTGATATTTTTCAAGATACTTTATACAGTTTAAAGTGACATGTAAATGCTTAAAAGTGCAGTAGGTGATCTTCCACAATGCTAGCAGCTTAGCATAAATCTTTGAATCACAGTGCCTCCCCTGCCGTCCAGAGCCACGCCTCCTGAAACAGGAGCCACGCGCACCTTAAAGATGACTGCAGAACCCTCTTCTCATAGAATAAAAAGACTCAGCTATGAATAATAATGAGAAACCAACGTGTCATCTTTGCACTTGCAGTTTTGCGCTACGTCGCCAATTTTGATACTGCCCCAAAAATCATTTTAAACCCGATATCTAAACTTAGCTGACAAAAGCTCAAAATTATCCAGTTTTTTCCACAATTAAAGCTGACAGGTGCTAACATTGTCTTAACTGATGCTCAACACACACAAATCTGCTAAAATCTCCAAAAAAGTACTCAAGGGTTTCGTGAACCTTTAAAATGGATTTTTAAAAAAAAATTAAAGCTGCTTTTATTCTTGCCGAAATAAAACAAATAAGACTTTCTCCAGAAGAAAAAATATTATTAGACCTACTGTGAAAAAATATTAGGGTGGCTAATAATTCTGACTTCAACTGTATATGCTGAGCACTGTATAAATTCCTTTTTTACTTCTATGAAAATAAATACAAACAGATATGGAAATGTGATGTGAAAATTTACAAGATGATGTCATCTAGTGCTCTTTTAGGCAGATTTTCTTTATTTTATATCGAAAATAGATGGCAACAGTGTTCAGAGCTGTATTCAAGTTCAGGTAGTCAGTTTGAATCATAATCGAGTAAAGTACAGTAAAGTAATACTTAGGTACAGTATTCAAGGGTTTTACACCAGTGGTGTCTCTGTCTCTTCCTCATACTGTCTTTCTTTTCAG
Seq C2 exon
CCATGCTAAAGGTGATCTCTTCAGTGCTGCAGTTTGGGAACATCACATTTCACAAAGAGAAGAACACGGATCAGGCCTCAATGCCAGATGACACCGCAGCACAGAAACTCTGCCACCTGCTGGGCATCAGCGTGCTGGAGTTTAGCCGTGCCATTCTCACTCCTCGCATCAAAGTAGGCCGCGAATATGTCCAGAAGGCCCAGACCAAGCAGCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000073732:ENSDART00000142155:10
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.102 A=NA C2=0.125
Domain overlap (PFAM):
C1:
PF0006316=Myosin_head=FE(6.5=100)
A:
NA
C2:
PF0006316=Myosin_head=FE(10.2=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTTTTGCTGGGAGGTGCAGAT
R:
GGTCTGGGCCTTCTGGACATA
Band lengths:
345-2254
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]