DreINT0100014 @ danRer10
Intron Retention
Gene
ENSDARG00000001014 | myh9b
Description
myosin, heavy chain 9b, non-muscle [Source:ZFIN;Acc:ZDB-GENE-030131-998]
Coordinates
chr3:5224714-5226991:-
Coord C1 exon
chr3:5226743-5226991
Coord A exon
chr3:5224927-5226742
Coord C2 exon
chr3:5224714-5224926
Length
1816 bp
Sequences
Splice sites
5' ss Seq
CAGGTCAGA
5' ss Score
7.41
3' ss Seq
CTGTGTGTGTTTGTCCTCAGATG
3' ss Score
12.38
Exon sequences
Seq C1 exon
CTGGTGGACATGAAGAAGAAGATGGAGCAGGAGTCTGGCTCTCTGGAGTGTGCGGAGGAGAGCAGGAAGCGAGTGCAGCGGGATCTGGAGGCCGTGTCTCAGCGTCTGGACGAGAGAAACGCCGCCTTCGACAAGCTGGACAAAACCAAGACCCGCCTGCAGCAGGAGCTGGACGACATGCTGGTGGACCAGGACCATCTGCGGCAGATCGTCTCCAACCTGGAGAAGAAGCAGAAGAAGTTTGACCAG
Seq A exon
GTCAGAACCAGCACTCGCTAAACACCTGGTTCATATTACGATCTCACTTCAGGTTTATTATATTAAAGATATTCAGAAAGTACAAACAGCGGAGACTAACACACAAACCCCACCAGCTGACCAGTCAATAAAGTGTACTAATGCAGCGGTGTCCAAACTCAGTCCTAGAGGGCCGGTGTCCTGCTGAGTTTAGCTCCAACTAGCTCCAACACACCTGCCAGGAAGTTTCTAGTATATCTAGTGAGCTTGATTAGCTGATTCAGGTGTGTTTGATTAGGGTTAGAGCTAAACTCTCCAGGACACCGGCCCTTCAGGACTGAGTTTGGACACCCATGTACTAAAGTATCTGTTAACAAAAAAAAGAACTCTCTTCAGATACACATTTCAGTTCAGTTTTAGTGGTCTGGGAAGCTCAGCGTCTTGAGGAAGACACAAAACCAGACTTATGATGCACAGGTATAGCTTTGAAAATAGCCAAAAACACATTTTATTCATCAAAATGATTTTTTTAATGCCAAAATTCATGTTTGTAAATTATATTTTTGTAAATTTCTACTGTAAATATCTCAAAACTCAATTTTAGATAAGTAATGTCCACTTTAAAGCTGAATTTCTCAATATCTATTTTTTATTTTGCACCCTCAGATTCATGATTATGTAATTATATTTCAGGCAAATATTGTCCAGTCATAACAAGCAAAATACACAACTGGAAAGATTTTTTTATGTAACTTTTAACTGATGCCTAAATAAAGACACCTGTGACTGGATTTGTGGTCAAGGGTCACATTTAAGAAAAACAGCACAACAAATATTAATATACAGTTTCTGTGCTTTTTTTTAAATATTGAAATTTTTTTAGTCCTTTTATGTTTAGTTATTACATTTTCATGAATATATAAATTGTTCTTGTGCGAGCTGTTTACAATAATTAAATAGATTTATTTTATTTATATATGTTTGTTTTACACGTTTTCAAAAATGTTTCAAAAAGTGTTTTAACATGTAGTACGAGTATTTTTCAAACATAAATGTATTATTTTTTTATTTTCTATTATTGTTTATTTTAGTTTTCTTACTAAAACGTGACAGTTTTTTCTTCATATATTATCTTGATTCATACTAAGTTGTTGTGTTTTTGTTGTTTTTATTCATTTAAAAATCTAATTTATAGTAAATGAAACAATTTATCAGGTATGGTGAGTTTTTAAAACAATTACATTGATTTCAAAATAAGTTTCTTATATTGATGTGTATTTAAAATATGTACCTTTCTCTAGTATTATCAAACGTTTTATTATTATTATTATTATTATTATTATTATTATAGTTTTTTTGCACATGCTCTTTCGATCAAAAGTTTTTAACATTTATTAAATATAAAAGAATTGTTTAATGGTTATTACCATTTTAAATAACGGCTTTATAATTGGATGCAGGTTCAAATAAAACTATTACTCCAGTCATTATTGCTTTTATTACTGTTACCATCAATAATAATAAAATTAATAATAATATTGATGATTAATATTAGAGGATTTCTGAATGATCATGTGAAGAGTGGAGTAATGAAGCTGAAAATTCATCTTTAAAATCCCTGGAATAAATGATTGAATTAAATGATAATCTGCTTTTGAATAATTATTTTATAGTGGATTAACATTTCACAATTTTACAACGTGTTCTGTATTAATGATTAAATAAATGCAGCCTGGTGGGCAGGAGAAGCTTAATTTTAAAACTTTTACAAATCATACTGACCCTAAACTCGGTACTGGTAGTGATTTCAGAGTGTTCTGTGTGTGTTTGTCCTCAG
Seq C2 exon
ATGCTGGCCGAGGAGAAGTCGATCTCTGCCAGATACGCTGAAGAGCGCGACCGTGCCGAGGCCGAGGCTCGCGAGAAGGAAACACGCATGCTAGCGTTAGCTCGTGAGCTGGAGACGCTCACCGATATGAAGGAGGAGCTCGACCGCACCAACAAACTGCTGCGCGCCGAGATGGAGGATCTGGTGTCATCTAAAGACGACGTGGGCAAGAGC
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000001014:ENSDART00000137105:30
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.590 A=NA C2=0.845
Domain overlap (PFAM):
C1:
PF0157614=Myosin_tail_1=FE(13.0=100)
A:
NA
C2:
PF0157614=Myosin_tail_1=FE(11.1=100),PF115593=ADIP=PU(37.7=60.6)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GAGTCTGGCTCTCTGGAGTGT
R:
TCATATCGGTGAGCGTCTCCA
Band lengths:
349-2165
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]