DreINT0100058 @ danRer10
Intron Retention
Gene
ENSDARG00000001993 | myhb
Description
myosin, heavy chain b [Source:ZFIN;Acc:ZDB-GENE-081124-1]
Coordinates
chr6:40750116-40750512:-
Coord C1 exon
chr6:40750347-40750512
Coord A exon
chr6:40750241-40750346
Coord C2 exon
chr6:40750116-40750240
Length
106 bp
Sequences
Splice sites
5' ss Seq
AGCGTATGT
5' ss Score
6.12
3' ss Seq
TCATGTTTTTGCATATACAGAGG
3' ss Score
5.41
Exon sequences
Seq C1 exon
GTCTTGGCCGAATGGAAGCAGAAGTATGAGGAGACCCAAGCAGAGCTGGAAGGAGCCCAGAAGGAGGCTCGCTCACTTAGCACGGAACTTTTCAAAATGAAAAATTCTTATGAGGAGACTTTGGATCATCTAGAAACTCTTAAGAGAGAGAACAAGAACCTTCAGC
Seq A exon
GTATGTACAATTAAGTGCAGAACTATATGTTGTTGTTTTTTTTTTTGCTTTTTAGACGTAAATAGTGCATTTCACCCAATTGTTGGTCATGTTTTTGCATATACAG
Seq C2 exon
AGGAAATCACAGATTTAACCGAACAGCTTGGCGAGACTGGAAAGACCATCCATGAGCTGGAAAAAGGAAAGAAAACTGCAGAAATCGAAAAATCTGAAATCCAAGCTGCTCTTGAAGAAGCTGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000001993:ENSDART00000156295:32
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.536 A=NA C2=1.000
Domain overlap (PFAM):
C1:
PF0157614=Myosin_tail_1=FE(6.4=100),PF0003816=Filament=FE(17.8=100),PF045827=Reo_sigmaC=FE(39.9=100)
A:
NA
C2:
PF0157614=Myosin_tail_1=FE(4.8=100),PF0003816=Filament=FE(13.3=100),PF045827=Reo_sigmaC=PD(24.6=81.0)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CAAGCAGAGCTGGAAGGAGC
R:
TCAGCTTCTTCAAGAGCAGCT
Band lengths:
254-360
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]