BtaINT0097072 @ bosTau6
Intron Retention
Gene
ENSBTAG00000018204 | MYH1
Description
Bos taurus myosin, heavy chain 1, skeletal muscle, adult (MYH1), mRNA. [Source:RefSeq mRNA;Acc:NM_174117]
Coordinates
chr19:30115252-30115629:-
Coord C1 exon
chr19:30115464-30115629
Coord A exon
chr19:30115377-30115463
Coord C2 exon
chr19:30115252-30115376
Length
87 bp
Sequences
Splice sites
5' ss Seq
AACGTGAGT
5' ss Score
9.54
3' ss Seq
TAAATGTTTTTCACCAACAGAGG
3' ss Score
7.41
Exon sequences
Seq C1 exon
ATCCTATCAGAATGGAAACAGAAGTATGAAGAGACTCATGCTGAACTTGAAGCCTCCCAGAAGGAGTCTCGCTCCCTTAGCACAGAGCTGTTTAAGATCAAGAATGCCTATGAGGAGTCCTTAGACCAACTGGAAACCTTGAAGCGGGAAAATAAGAACTTGCAAC
Seq A exon
GTGAGTACACTTTACCTTTTCTCCAACAAAGAATTTGAAAGAATGTTTTCCTCACTGAATGAATTTTTAAATGTTTTTCACCAACAG
Seq C2 exon
AGGAGATCTCTGACCTCACGGAGCAGATCGCAGAAGGAGGGAAACGTATCCATGAACTGGAGAAAGTAAAGAAGCAAGTGGAACAAGAGAAGTCTGAAATTCAGGCTGCTTTAGAGGAGGCAGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000018204:ENSBTAT00000009327:31
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.339 A=NA C2=0.452
Domain overlap (PFAM):
C1:
PF0157614=Myosin_tail_1=FE(6.4=100),PF0003816=Filament=FE(19.6=100),PF045827=Reo_sigmaC=FE(36.7=100)
A:
NA
C2:
PF0157614=Myosin_tail_1=FE(4.8=100),PF0003816=Filament=FE(14.6=100),PF045827=Reo_sigmaC=PD(23.3=83.3)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCTGAACTTGAAGCCTCCCAG
R:
CGTTTCCCTCCTTCTGCGATC
Band lengths:
173-260
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development