Special

MmuINT0103199 @ mm10

Intron Retention

Gene
ENSMUSG00000056328 | Myh1
Description
myosin, heavy polypeptide 1, skeletal muscle, adult [Source:MGI Symbol;Acc:MGI:1339711]
Coordinates
chr11:67219639-67220018:+
Coord C1 exon
chr11:67219639-67219804
Coord A exon
chr11:67219805-67219893
Coord C2 exon
chr11:67219894-67220018
Length
89 bp
Sequences
Splice sites
5' ss Seq
AACGTGAGT
5' ss Score
9.54
3' ss Seq
TATTTCCTTTTTCCTAACAGAGG
3' ss Score
9.25
Exon sequences
Seq C1 exon
ATCCTGGCAGAATGGAAACAGAAGTATGAGGAAACCCATGCTGAACTTGAAGCCTCCCAGAAGGAGTCCCGCTCTCTCAGCACAGAGCTGTTCAAGATTAAGAATGCTTATGAGGAATCTTTAGACCATCTCGAAACCTTGAAAAGGGAGAATAAGAATTTGCAAC
Seq A exon
GTGAGTGTCTCTCCCATTTTCCTTAATGAGAGATTTGAAGGAACACCCCACAATCTCTATACACATTTCTATTTCCTTTTTCCTAACAG
Seq C2 exon
AGGAGATTTCTGACCTCACTGAACAGATTGCAGAAGGAGGGAAACGCATCCATGAACTGGAGAAAATAAAGAAGCAAATTGAGCAGGAAAAATCTGAGCTCCAGGCTGCTTTAGAGGAAGCAGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000056328:ENSMUST00000124516:32
Average complexity
IR
Mappability confidence:
NA
Protein Impact





	

	
ORF disruption upon sequence inclusion





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.411 A=NA C2=0.429
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
PF0157614=Myosin_tail_1=FE(6.4=100),PF0003816=Filament=FE(19.0=100),PF045827=Reo_sigmaC=FE(38.5=100)

							
A:
NA

							
C2:
PF0157614=Myosin_tail_1=FE(4.8=100),PF0003816=Filament=FE(14.2=100),PF045827=Reo_sigmaC=PD(23.8=81.0)

						

					

				








    
Main Inclusion Isoform:
NA





     
                   









    
Main Skipping Isoform:
ENSMUSP00000018637





     
      









    
Other Inclusion Isoforms:
NA
          









    
Other Skipping Isoforms:
ENSMUSP00000075147, ENSMUSP00000117569
  



Associated events






Other assemblies


mm9




Conservation


Human
(hg38)
chr17:10497194-10497286 
ALTERNATIVE
HsaINT0107444
(MYH1)
Human
(hg19)
chr17:10400511-10400603 
ALTERNATIVE
HsaINT0107444
(MYH1)
Rat
(rn6)
chr10:53759802-53759889 
Cow
(bosTau6)
chr19:30115377-30115463 
LOW PSI
BtaINT0097072
(MYH1)
Chicken
(galGal4)
chr18:436022-436193 
Chicken
(galGal3)
chr18:440669-440840 
GgaINT0127800
([1])
Zebrafish
(danRer10)
chr24:41032232-41032334 
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
CCCATGCTGAACTTGAAGCCT

				
R: 
GGATGCGTTTCCCTCCTTCTG

				
Band lengths: 
183-272

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Ribosome-engaged transcriptomes of neuronal types
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types





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