DreINT0100868 @ danRer10
Intron Retention
Gene
ENSDARG00000074622 | myo5ab
Description
myosin VAb [Source:ZFIN;Acc:ZDB-GENE-050411-72]
Coordinates
chr25:7443348-7443775:-
Coord C1 exon
chr25:7443621-7443775
Coord A exon
chr25:7443435-7443620
Coord C2 exon
chr25:7443348-7443434
Length
186 bp
Sequences
Splice sites
5' ss Seq
AAGGTCAGA
5' ss Score
6.8
3' ss Seq
CCGTCTGTCTGTGTTTGCAGAAA
3' ss Score
10.56
Exon sequences
Seq C1 exon
ACCTCAAACCTCGAGGTGTAGCTGTGAATCTGGTGCCAGGTCTGCCTGCTTATATTCTCTTCATGTGTTTGCGACATGCCGACTACGCCAACGATGACCTGCGGGTGTCCACCCTCCTCAACACCTCCATCAACAGCATCAAGAACACACTGAAG
Seq A exon
GTCAGAAACTCACGGAAAAATAATCCACTAAGATGAAAACACCAAGTGAAACATCTTTACAAGCTTCACATTGTCCATATACATGCAGTGGACCCTTTAAAACTGTTAGGTATTTGTGGATGTATACTGTTTTTATAGTATACAAGTTCACCCTTTTCAATGAGAGCCGTCTGTCTGTGTTTGCAG
Seq C2 exon
AAACGTGGAGATTTTGAGAGCATTTCATTCTGGCTGGCGAACACCTGTCGCTTCCTGCATTGCCTGAAGCAGTACAGTGGAGAAGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000074622:ENSDART00000089034:38
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCTCGAGGTGTAGCTGTGAAT
R:
CTCTTCTCCACTGTACTGCTTCA
Band lengths:
234-420
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]