DreINT0101035 @ danRer10
Intron Retention
Gene
ENSDARG00000099871 | myo7aa
Description
myosin VIIAa [Source:ZFIN;Acc:ZDB-GENE-020709-1]
Coordinates
chr18:3150418-3150753:+
Coord C1 exon
chr18:3150418-3150545
Coord A exon
chr18:3150546-3150626
Coord C2 exon
chr18:3150627-3150753
Length
81 bp
Sequences
Splice sites
5' ss Seq
GAGGTCAGA
5' ss Score
5.24
3' ss Seq
GAGTGTTGTTCTCCCTGCAGAGA
3' ss Score
9.26
Exon sequences
Seq C1 exon
GTAACCAAGCGTTTAAATGACGGGGAATACACTGTTCATGGAAACAGCATGTTGGAGGATCGACCCACGTCCAATCTGGAGAAACTGCACTTTATCATCGGCAACGGCATCCTGCGTCCAGGCCTGAG
Seq A exon
GTCAGACGGTGGATATCTGCTGTAAATATCAGGTTATGATTGTAAAACACTGATTGATACTGAGTGTTGTTCTCCCTGCAG
Seq C2 exon
AGATGAGATCTACTGTCAGATCTGTAAACAGCTGAACCAGAATCCGTCTAAAAGCAGTCACGCTCGCGGCTGGATCCTCATGTCTCTGTGCGTCGGCTGCTTTGCTCCCTCTGAGAAGTTTGTCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000099871:ENSDART00000166980:25
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.163 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0078412=MyTH4=PU(18.9=46.5)
A:
NA
C2:
PF0078412=MyTH4=FE(39.6=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CAGCATGTTGGAGGATCGACC
R:
AGAGACATGAGGATCCAGCCG
Band lengths:
171-252
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]