DreINT0101089 @ danRer10
Intron Retention
Gene
ENSDARG00000044632 | myo7ab
Description
myosin VIIAb [Source:ZFIN;Acc:ZDB-GENE-091110-4]
Coordinates
chr21:22259754-22260230:+
Coord C1 exon
chr21:22259754-22259923
Coord A exon
chr21:22259924-22260039
Coord C2 exon
chr21:22260040-22260230
Length
116 bp
Sequences
Splice sites
5' ss Seq
CAGGTGAGC
5' ss Score
9.6
3' ss Seq
GTTTTTGTTTGTTTTTTCAGCTG
3' ss Score
10.15
Exon sequences
Seq C1 exon
AGGTGGAAAACTGCAGGGACAGAGCTTTACCCTGGCCACTATTAAAGGAGATGAGTACACCTTCACATCCAACAACGCTGAAGACATCCGGGATCTGGTTGTGGCTTTTCTAGAGGGCCTGCGTTCCAGGTCAAAGTTTGTGGTGGCAATGATGGACAGCCATTATCCAG
Seq A exon
GTGAGCTCACAGTTGGGTTTAACTTTAATAACATTGTATTTGATTTGGGCCCCAAAACAATTAGGTTGAGCATAATTAATAAAACATCTAAAAAATGTTTTTGTTTGTTTTTTCAG
Seq C2 exon
CTGGGCAAGATGCTTCGTTCCTGAGATTCTCAAAGGGAGATCTGATCTTTCTGGATGAGCACACTGGAGAACAGGTCCTGACTTCAGGCTGGGCTCATGGGGTCAATGACCGAACCAAGAAGAGAGGAGACTTTCCTGCGGACAATGTCTATGTGCTGCCCACCATCACCAGGCCACAGTATGATATTGTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000044632:ENSDART00000148019:32
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.062
Domain overlap (PFAM):
C1:
PF0001823=SH3_1=PU(6.0=5.2)
A:
NA
C2:
PF0001823=SH3_1=PD(92.0=71.9)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGACATCCGGGATCTGGTTGT
R:
GATGGTGGGCAGCACATAGAC
Band lengths:
256-372
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]