DreINT0106213 @ danRer10
Intron Retention
Gene
ENSDARG00000009341 | nrap
Description
nebulin-related anchoring protein [Source:ZFIN;Acc:ZDB-GENE-060616-224]
Coordinates
chr12:30486011-30486615:+
Coord C1 exon
chr12:30486011-30486208
Coord A exon
chr12:30486209-30486510
Coord C2 exon
chr12:30486511-30486615
Length
302 bp
Sequences
Splice sites
5' ss Seq
GACGTAAGT
5' ss Score
10.93
3' ss Seq
ATATATACTATATACTACAGAAC
3' ss Score
4.12
Exon sequences
Seq C1 exon
GTTGCTTATAAAAAGGATTCTAAGGAAACTCAATCCAAGTGCCACCTGCCTCAGGACATGGTGAATCTGAGTCATGCTAAGAAGGCTCAAGCTCTGGCCAGTGATCTGGAATATAGGAAGAAGTTACATGATTATACTCTCCTGTCTGATGACATGAAAGTGCAGCAAGCTAAAAAAGCCTACAAACTGCAGAGTGAC
Seq A exon
GTAAGTAATGCAAGAACAGAGCGGAATGATACTCATAATGTAACTCTATGTGTTTGCTAAATACTCTAAATCAGTGTTTCCCAACCCTGTTGCTGAAGGCACACCAACAGTACACATTTTCAACCTCTTCCCAATCAAACACATCTGAATCAAGTCATCAGAACATTAGAAGAGACTCCAAAACCTGAAATTAATGGGCCAGATAAGGGAGACACCCAAAACATGTACTGTTGGTGTGCCTTCAGGAACACGGTTGGGAAACACTGCTCTAAATATTACTTTATATATACTATATACTACAG
Seq C2 exon
AACCAGTACAGATCAGACCTGAACTGGATGAAAGGAGTTGGATGGGAGGCCGATGGATGCCTAGATGTAGAACAGGCCAAGAAAGCAGGAGAACTCTTCAGTGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000009341:ENSDART00000124920:20
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.167 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0088013=Nebulin=WD(100=39.4),PF0088013=Nebulin=PU(55.2=24.2)
A:
NA
C2:
PF0088013=Nebulin=PD(37.9=31.4),PF0088013=Nebulin=PU(55.6=42.9)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TTCTAAGGAAACTCAATCCAAGTGCC
R:
CTCACTGAAGAGTTCTCCTGCT
Band lengths:
286-588
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]