RnoINT0103040 @ rn6
Intron Retention
Gene
ENSRNOG00000016714 | Nrap
Description
nebulin-related anchoring protein [Source:RGD Symbol;Acc:1306731]
Coordinates
chr1:277146887-277147518:-
Coord C1 exon
chr1:277147321-277147518
Coord A exon
chr1:277146992-277147320
Coord C2 exon
chr1:277146887-277146991
Length
329 bp
Sequences
Splice sites
5' ss Seq
GAGGTGAGT
5' ss Score
10.03
3' ss Seq
TGGTGACACTTCTCCTGCAGCTG
3' ss Score
7.38
Exon sequences
Seq C1 exon
GTGGAATATAAGAAAGGGTTTGAGGAGAGTAAAACCCACTTCCACTTGCCCATGGACATGGTGAACCTCAGGCATGCTAAGAAGGCCCAGGCCCTCGCCAGCGACCTCGACTACAGAAAGAAACTGCATGACTACACAGTGCTGCCTGAAGATCTGAAAACTCAGTGGGCCAAGAAAGCCCACGGGCTCCAGAGTGAG
Seq A exon
GTGAGTGAACACTGGCTGATGGGGAGGGGCTTGGGGCCAGCGTGAAGGTCAAGGTGTGCTGGAAACAAGAGCTGAGGAGAAACATCTTGGAAATACCGGTCAGCAATATGAGAGACCATTTTTGGCAAGGCCAAAGTGCTCCAACACTACGGTGAAATGTGTCCATCTCCATCTCAGGGAGCACCCGGTGGCTTGCCCTCCCGAAGGAAAAGCCACTGAGGATAATTGGGGAGTAGCTTTCTGGGAGGACAAACTTGTATTCCCCCTGTGAACTTGGTTGTGTCAGCCTGTGTGTGTCGGACTAGAGCTTGGTGACACTTCTCCTGCAG
Seq C2 exon
CTGCAGTACAAAGCTGACCTGGCATGGATGAAGGGTGTCGGGTGGCTGACGGAGGGGAGTCTCAACCTGGAGCAGGCGAAGAAGGCTGGACAGCTCGTCAGCGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000016714:ENSRNOT00000038017:18
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.057
Domain overlap (PFAM):
C1:
PF0088013=Nebulin=PD(39.3=16.7),PF0088013=Nebulin=WD(100=39.4),PF0088013=Nebulin=PU(55.2=24.2)
A:
NA
C2:
PF0088013=Nebulin=PD(37.9=31.4),PF0088013=Nebulin=PU(55.2=45.7)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTGGAATATAAGAAAGGGTTTGAGGA
R:
CAGCCTTCTTCGCCTGCTC
Band lengths:
286-615
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]