DreINT0108825 @ danRer10
Intron Retention
Gene
ENSDARG00000040482 | osbpl3b
Description
oxysterol binding protein-like 3b [Source:ZFIN;Acc:ZDB-GENE-090109-1]
Coordinates
chr16:21576190-21578158:+
Coord C1 exon
chr16:21576190-21576327
Coord A exon
chr16:21576328-21578094
Coord C2 exon
chr16:21578095-21578158
Length
1767 bp
Sequences
Splice sites
5' ss Seq
CAGGTAGGA
5' ss Score
9.79
3' ss Seq
TCTTTGCTTTTGTCCTCCAGGTC
3' ss Score
11.87
Exon sequences
Seq C1 exon
GTTTATATTGCTACATTCGCCGTGTCTGGCTATGCTTCCAGTTATTACAGAGCAGGAGGAAAACCGTTTAATCCAGTGTTGGGTGAAACCTATGAGTGTGATCGTCCTGACAAAGGCTTTCGTTTTGTCGCTGAGCAG
Seq A exon
GTAGGAATTTTTCTGTAAACATTGTAACTGATGAACAGGAATGTGGATATTTTCAGGGCTTCTGTAAAAAACTAACATGATAAGGTTTAGGGCTATTGTTACATCAAGAAGACTGATAGAATTACATAAAATAAAGGTTTCTTGGATTAAAGGTGCAGTTACAGTGAAACACTATTGATATTTGAAATCGACATCCTAACAATCACACAACTAGCCAAATGAAACACATCCTTCCCTTTATCACTTGGCCCTCATATTTACTACAAACACACTATACAACACAGAAAGTAAAAAATAGCTGGAGCACCAGACTAAAATATCCTGGCTTTATGAAACAGCAAAGCCAAATATTTCATAAATGTTGAGCAGAAACATAGAGTGTTGTGGTGCTCCTTTTGATTGCTCTGATACATCTGTAATAACTGTTTTTATTTGATAATCCACTTTTATAAACACAATGTTTGTTTTTCAACACATCTATAGACCATTTTGAGGGATGTAAACAAAAACAAGGGTCCCAACATATTTCCTGTTTTACATTTTTCATTTCTCTAGCTTCCAAAAATCCAAAAGGAGCTACATATTGATAGATAATGTTATGACACCTGTTTTAACATTAAGTTATAATTGAATTGTCTCTTGTTACAGTTATGAAATAGTTTGACAACAAGCAGGAAATATTTATGGGCCAATGACATCACCACATTGAAATGGTCTATAGAACAGAACAGAGCAAGTATTCACAAGATGCCATAATAAACATTAATATTGAAATTCTACAATTTTTCTATGATCTATATTTTTTATTTAATATTTGAGTTCTTACTTGAATGTTTAAGATAATACTTAGTAATAATGAATGTTAGTGATGAGGAATATACTGTCATATCTTTATTCTTTTTTTAAATTCTTTTTTTTTAAGTTCAATACAGATTTTAAGACATTTTTAAACATAATATTTTTAAGAGCTAATTTCTTTTTTCTTTGCCATAATTACACTCACTCACTCAATATCTTTTCTGTATTTTACAAAAAATAAAACTTTCTTAATGAAAAATAAATAAATAAATAAATTATAATAAAAAAGTCTATTGGAAAAGGCTATATCCATGTTTCACATATTTGTTTTTACCATATGGTTACTGAAAAAAGTTAAGCCTCATTCATGTTATGAGTGACTCGCAGCAACAAGGGACTGTTCATTTTAATGAAGAGTAAGTGACTTCTGGCAACCTCCGTCTATGCAAGCAACAGCGACCATTGGCAACCAGGTAGGCGTGTCGAGTGATCCTATCAGCTCCTGCTGAGGCCGGTTGTATTCTCCCCGCTATAGACTTACATAGACCTGCATTTGCAACAGATCGCCACCCAGAGCAACAGGTAGCTACAAAGTCGCTGCTAGTGTGAATGAGGCATTAGAAAGAAAGGGTACACTTAGGGCTTGGCGATTAATCGTAAAGTAATGGAAATCAGCATTCAGAACCTATAATCGATCGAATTTCGGAGAGGGCAATAAAAATAAAATGAATAAATAAATAAAAGAATCATTTAAAATGTTTAATGTTTAAAAATCAAGTTAAAATATTAAATTAATTGAAATTTGAAGATTAGGCCAAATCACCTAGCCTTACTGTAGGTACACCCAAGTCGGAATACTCAAAAATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTCAATCTCACAGCTTCTTTGCTTTTGTCCTCCAG
Seq C2 exon
GTCAGCCATCACCCCCCCATATCATCATGTCATGCCGAATCTAAAAACTTCATCTTTTGGCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000040482:ENSDART00000145886:16
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0123713=Oxysterol_BP=FE(13.0=100)
A:
NA
C2:
PF0123713=Oxysterol_BP=FE(6.1=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATATTGCTACATTCGCCGTGTCT
R:
CTTGCCAAAAGATGAAGTTTTTAGAT
Band lengths:
198-1965
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]