DreINT0111795 @ danRer10
Intron Retention
Gene
ENSDARG00000051915 | pde5ab
Description
phosphodiesterase 5A, cGMP-specific, b [Source:ZFIN;Acc:ZDB-GENE-060824-4]
Coordinates
chr7:57271642-57272052:+
Coord C1 exon
chr7:57271642-57271750
Coord A exon
chr7:57271751-57271967
Coord C2 exon
chr7:57271968-57272052
Length
217 bp
Sequences
Splice sites
5' ss Seq
ACAGTAAGT
5' ss Score
9.49
3' ss Seq
TCCTCTTTTCTTTTTTAAAGAGT
3' ss Score
7.96
Exon sequences
Seq C1 exon
AGAATGTGACGTCAACAAGATTAACTACATGTACGCTCAGTATGTCAAAAACACTATGGAGACACTGAACATCACAGATACCACCAAAGATCAGCTATTTTCATGGACA
Seq A exon
GTAAGTGCTCAGGGGAATGCTGCAATGGAAAGTAAGCTGCCATAGGGTCATCCCACTCTGGGTCACCCTGATGTTTCTGAGATTTCACAGGCCTCTTATTCAGACATTCTCCTCCCCCATTTTGTGTATGAATTGTCAGTTTTCCCAAAGATGCTTTACTCTGAACTAATTTTAACTCTATGGCATTCATGATTTTTTCCTCTTTTCTTTTTTAAAG
Seq C2 exon
AGTGAAGATCCACAGTCCACGAACTGTCAAATCAAAAGTCTGCTGTGCACACCAATAAGGAATGGGAAGAAAGACAAAGTAATAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000051915:ENSDART00000138188:8
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0159021=GAF=FE(22.9=100)
A:
NA
C2:
PF0159021=GAF=FE(17.8=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGAATGTGACGTCAACAAGATTAACT
R:
ACTTTGTCTTTCTTCCCATTCCT
Band lengths:
189-406
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]