MmuINT0117027 @ mm10
Intron Retention
Gene
ENSMUSG00000053965 | Pde5a
Description
phosphodiesterase 5A, cGMP-specific [Source:MGI Symbol;Acc:MGI:2651499]
Coordinates
chr3:122794260-122795321:+
Coord C1 exon
chr3:122794260-122794368
Coord A exon
chr3:122794369-122795233
Coord C2 exon
chr3:122795234-122795321
Length
865 bp
Sequences
Splice sites
5' ss Seq
ACAGTAAGT
5' ss Score
9.49
3' ss Seq
GTCTTGGCTTTGTTCTTTAGAAT
3' ss Score
8.37
Exon sequences
Seq C1 exon
GGAGCAAGATGCAAACAAAATCAACTACATGTATGCTCAGTATGTCAAAAACACGATGGAGCCACTTAATATCCCAGATGTCACCAAGGACAAAAGATTTCCCTGGACA
Seq A exon
GTAAGTAACCTAATTTTAGACCTGACAATCACAGGCTGGTCTCCCTTCAGACCCAGACTTACACAGCTGCTTGGTGTCTGAAGGACTAGGCTGGAAACTGGCTGTTTAAAGGGAAGGATCTCTGTTTTTCCTGCTCGAGGTGGGGGATGGTAAAGAACCTCTATGTGTGTTGGGAAGGCTGTGCTGTGTGTTTTTTGTATAGTGACAAACTAGCATGTGCAAATTGAGGTAATTTAAAGAACACGGTGTCAGCGACTCTCCTCAGATGGAGTTCCTGACTTTATCTGTAAACATTACGGTCCGTGTGGTTTGGCTGCACACCACTGGCCGTCTTCTTAGCGAGCTGCTCCTTGGGAAGATGGGCATCCACTTGCCTTGACAGCGAAGTTATCACAGAGATGTGTTCCACAGACACTGAACACTTTGAATTCCTGTGTTTTCGTTAATCTTTCAGAAAACACTAGCCTGTGACACAGCTGCCTTGATTCTCTTGCCTGCACACTTGGGACAATGTTGTCAGACTGGGAATGTAGGCTTCAAATATGATGGCATTATTAAGAATGCCAATATTGTCATGTGTTGTTTCCAGGCTTTGGATTCTGCCTGCCTTGTAGAATGGAAACCACTACCTCACCAGGACAGCAGACACTGAATAAATATTTGACTTAGGTTTACTATAAACTCTTTGGAGAGTTTAAATAAAGGACATTTGGTATTTTTCTGATAAAGACTGACTTTATTGCTCATTGTCTGTAGCTGATACAAGATGAGCATGTGCGTTATTTCATGTGTGTTCTACAGTTAATAGGATGTTCGTGGGACCAGTTATTTCCGTTGTTTTAGTGGTCTTGGCTTTGTTCTTTAG
Seq C2 exon
AATGAAAACATGGGACACGTGAACACGCCCTGCATTGGAAGTTTGCTTTGCACCCCTATAAAAAATGGGAAGAAGAACAAAGTCATAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000053965:ENSMUST00000066728:8
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.054 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0159021=GAF=FE(22.8=100)
A:
NA
C2:
PF0159021=GAF=FE(18.4=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCAAGATGCAAACAAAATCAACT
R:
TGACTTTGTTCTTCTTCCCATTTT
Band lengths:
191-1056
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types