RnoINT0108890 @ rn6
Intron Retention
Gene
ENSRNOG00000014443 | Pde5a
Description
phosphodiesterase 5A [Source:RGD Symbol;Acc:620995]
Coordinates
chr2:226973749-226974826:+
Coord C1 exon
chr2:226973749-226973857
Coord A exon
chr2:226973858-226974738
Coord C2 exon
chr2:226974739-226974826
Length
881 bp
Sequences
Splice sites
5' ss Seq
ACAGTAAGT
5' ss Score
9.49
3' ss Seq
GTCTTGGTTTTGTTCTTTAGAAT
3' ss Score
8.91
Exon sequences
Seq C1 exon
GGAGCACGATGCAAACAAAATCAATTACATGTATGCTCAGTATGTCAAAAACACGATGGAGCCACTTAATATCCCAGATGTCACCAAGGACAACAGATTTCCCTGGACA
Seq A exon
GTAAGTAACCCGATTGTAGACCTGATGCTCACCGGCTGTTTTCCCTTCAGACCCACATGCACAGCTGCTTTTTGTCTGAAGGACTAGGCTGGAAACTGGCTGTTTAAGGGGGAGGATCTCTGGTTTTCCTGCTCGAGGGAGGGGATGGTAAAGTATGTTTATGTTTGCTGGGGAGGGTGTGCTGTGTGCTTCCGCTGACTCTTTGTATAGTGACAAACCAGCATGTGCAAGTTGAGGTGATTTAAAGGACATGGTGTCAGAGACTTTCCTCAGATGGAGTTCCTGATTTTATCTGTAAAAGTTAAGGTCCCTGTGGTTTGGATGCACGCCACTGGCAGTCTTCTTAGTGAGCTGCTCCTTGGGAAAGATGGGTATCCGCTTGCCTTGACAGCGAAGTCATTACAGAGATGTGTTCCGCAGACACTGAACGCTTTGAATTCGGTGTTTTCTTTAATCTTTCGAAAATGCCAGCCTGTGACACAAGGCTGTCTTGATTCTGTTGTCTGCATACTTGGGACAACTTTGTCAGACTATGGAAATGTAGGCTTCAAGAATGGTGGCGTTATTAAGAATTCCAATACTGTAATATGTTGTTTTAGGATTTGAATTCTGCCTGCTTTGTGAATTGAGACCTCTACCTCACCAGGGTAGCACACACTGAATAACTATTTGACTTGCTTTTACTATAGACTCTTTGAAAGGTTTAAGTAGAATAAAGGACACTTGGTATATTTTTCTAATAAAGATTGACTTTGCTGCTCATCGTCTGTGGCTGATCTAAGATGAGCACATGAGTTATTTCACATGTACCTTACAGGTAATGCGATATTTGTGGGACCAGCCATTACTGTTGTTTTAGCGGTCTTGGTTTTGTTCTTTAG
Seq C2 exon
AATGAAAACATGGGCCATATAAGCACGCACTGCATCGGAAGTTTGCTTTGCACGCCTATAAAAAATGGGAAGAAGAACAAAGTCATAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000014443:ENSRNOT00000019638:8
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.027 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0159021=GAF=FE(22.8=100)
A:
NA
C2:
PF0159021=GAF=FE(18.4=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCACGATGCAAACAAAATCAAT
R:
TGACTTTGTTCTTCTTCCCATTTT
Band lengths:
191-1072
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]