DreINT0124773 @ danRer10
Intron Retention
Gene
ENSDARG00000029372 | rasa4
Description
RAS p21 protein activator 4 [Source:ZFIN;Acc:ZDB-GENE-060526-349]
Coordinates
chr5:58691613-58691959:+
Coord C1 exon
chr5:58691613-58691766
Coord A exon
chr5:58691767-58691894
Coord C2 exon
chr5:58691895-58691959
Length
128 bp
Sequences
Splice sites
5' ss Seq
CTAGTAAGC
5' ss Score
5.2
3' ss Seq
TGTTTTCATTTATCCTTTAGCTG
3' ss Score
8.2
Exon sequences
Seq C1 exon
GGAAACTGGCCTGATTTCATCATGCTGATTGATGAGACCACTACAGCGGAGAGCAGACAGGAAGTAGCTAATAATCTAGTAAAGCTGTTTTTGGGTCAAGGGCTTGTCAAGGAGTTCTTGGACGTTCTGTTCAAACTGGAGCTGGACAAGACTA
Seq A exon
GTAAGCAAAGATCTCTAATGTATTTGTGTGTGAAATATTGTTTTTCTTTTTGTATTTATTTCTCCATTACTTGCACTATGATGCTACGTACGTAATAATGCTACGTTTTGTTTTCATTTATCCTTTAG
Seq C2 exon
CTGAACCCAACACACTCTTCCGCAGTAATTCATTAGCATCTAAGTCTATGGAATCCTTTTTGAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000029372:ENSDART00000097374:10
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0061614=RasGAP=PU(6.8=25.0)
A:
NA
C2:
PF0061614=RasGAP=FE(11.1=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACTGGCCTGATTTCATCATGCT
R:
AAAAGGATTCCATAGACTTAGATGCT
Band lengths:
210-338
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]