DreINT0124796 @ danRer10
Intron Retention
Gene
ENSDARG00000036257 | rasal2
Description
RAS protein activator like 2 [Source:ZFIN;Acc:ZDB-GENE-070912-180]
Coordinates
chr2:35861103-35862278:+
Coord C1 exon
chr2:35861103-35862063
Coord A exon
chr2:35862064-35862146
Coord C2 exon
chr2:35862147-35862278
Length
83 bp
Sequences
Splice sites
5' ss Seq
CAGGTGAAG
5' ss Score
6.66
3' ss Seq
TGTTTGCTTGTGTCAATAAGGTC
3' ss Score
4.17
Exon sequences
Seq C1 exon
TGAAATGAGGAGCATCAAATCACCTGTCCAAGAGCACATGGAGGCTTTAGTCAGAGGAAAGCATCCTTTACTGGGTCAACAGTCGTCCGCACACAGCATGAGCTTCTCCGACAAAGAGGAAAGGGACAGTCCGCTCCCGAATGGACGCAGTATATCTTTAATGGACTTGCAGGACTCTTACCTTGCTCAGGGGCATCCAGGTCCCAACTCGCTCAATGAAGCCTCATCTAGGTTAGGCAGAGTAGGCTCTCAGGCCTCCATCGGCCCTGTCCCTCCTCCACACCTCCACCAGCCTCCAGTCCACCCGAAAGTTCCACAGCTGAGGGATAACTTACCCCAGAGTGCACCACAGGTGCGGCGCCCGATCCACCCATCCCTTAGCCAGCAGCGCAGCCTACAACCGCTGTCTTTCCAAAACCCTGTTTACCACCTCAGCAACCTGCACGCACAATCAACACACTCAACCCAGTCCACACACTCTGCCCAGTCCCTGCAGCCTGACTCTAGCTCAGAAAACCTGAGCACAGGGAGCTCTCGGAGCGCCAGTCCTAGTGCCTCAGGTGGCCGGGGAGCAACTCCCAGAGCCCGGATACCCTCCACAAGCAGCGTGGAGGAGGAATTTAGCCGAAGAAGCATCCAGGGACAGGAGACGCCGCCTCCAACGGCACGCTGGCACCCTCCCCTGGCAGACCAGCACTCGGGAGCCCAGGTGGTGGCAGTGCCAAGGCAGAGTACCACCGGTACGGCACATATAGTGAAAGTGGAGCAGCAGAGTCGAGGAGTTGGGTTGGTGGCAGCAAATGCTGGAGCACGAACCCCAAGGTCGCTCCCTCACAGCACGTCCATCCGCAGCGGCAGCAGCGCCAACACTGAACCCATACAGCAGAGCGGTGAACGATCAAGACAGCAGTCCACGTGCTCCAAAGATGGTTCAGTACCAGGAGGACGAGCCAACAAACAG
Seq A exon
GTGAAGACAGACTTGAGGAAATTTGAGTATGTTTACTTTATTGTCAAGTGTTTGAGCACTAACTGTTTGCTTGTGTCAATAAG
Seq C2 exon
GTCCAGTCACCTGTAGAGTCTGTCACCATGTCCCCAGTGGAGCGAACAGCTGCGTGGGTTTTGAATAATGGCCAGTATGAAGATGAGGAAGAGGAAGGGCAGAGTAAAGACGATGCCAAACATGTGGAGAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000036257:ENSDART00000113489:14
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=1.000 A=NA C2=0.977
Domain overlap (PFAM):
C1:
PF120043=DUF3498=FE(59.9=100)
A:
NA
C2:
PF120043=DUF3498=FE(8.1=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CGATCAAGACAGCAGTCCACG
R:
GGCATCGTCTTTACTCTGCCC
Band lengths:
183-266
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]