DreINT0124817 @ danRer10
Intron Retention
Gene
ENSDARG00000089933 | rasal3
Description
RAS protein activator like 3 [Source:ZFIN;Acc:ZDB-GENE-101130-3]
Coordinates
chr6:8248490-8248858:-
Coord C1 exon
chr6:8248725-8248858
Coord A exon
chr6:8248569-8248724
Coord C2 exon
chr6:8248490-8248568
Length
156 bp
Sequences
Splice sites
5' ss Seq
GAGGTAATG
5' ss Score
8.73
3' ss Seq
TTTTTTTTTATTCATTTCAGCCT
3' ss Score
8.56
Exon sequences
Seq C1 exon
AAAAGTGTGAACCTGATTATGTGCTCCAAGACCCTGTTCGGCTATCCTGGGCCCCCCCACAAGAAGTTCCCGTTTGGGACATTAGCAACTGCTTTCTTCAGGATGGACAGATCCTTATCCCACGTGAAGAGGAG
Seq A exon
GTAATGGACATCTAATACATTCAATAATGTCAAAAGATGAGGCGTTGTGCTTAACAGATTGAGATGCTGGATGCGTGCCAAAGTTAGTTTGAAAATGCTAATGGATTGATTGATGCTGCCAGTGTATGATTGTTTTTTTTTTTTTATTCATTTCAG
Seq C2 exon
CCTATGATGACAGTGCGGAACCGGGCCAGCAGTTGTCTCTTCAGCGTCAGTCTGCAGAACATAAGCGGAAGTCATGCTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000089933:ENSDART00000124643:2
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.378 A=NA C2=0.222
Domain overlap (PFAM):
C1:
PF0016924=PH=PU(9.0=33.3)
A:
NA
C2:
PF0016924=PH=FE(15.7=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGTGTGAACCTGATTATGTGCTCC
R:
CAGCATGACTTCCGCTTATGT
Band lengths:
210-366
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]