DreINT0124822 @ danRer10
Intron Retention
Gene
ENSDARG00000089933 | rasal3
Description
RAS protein activator like 3 [Source:ZFIN;Acc:ZDB-GENE-101130-3]
Coordinates
chr6:8240122-8240976:-
Coord C1 exon
chr6:8240878-8240976
Coord A exon
chr6:8240785-8240877
Coord C2 exon
chr6:8240122-8240784
Length
93 bp
Sequences
Splice sites
5' ss Seq
AAGGTATAG
5' ss Score
7.98
3' ss Seq
GTACTCTTGTGTTCTGAAAGGAC
3' ss Score
5.81
Exon sequences
Seq C1 exon
GTTATAAACTCAGAAAATAACCACTGCTTTGGGTGCACTTCAGCTGCAGAAAGAGACCGCTGGATTGAGGACTTGCGTCGTACGGCTCATCCAAATAAG
Seq A exon
GTATAGCATATCTTTCAATTTTAATATCCAATTTAAATTATATTATTTCTATTTATTCTAATACTATCTATTTGTACTCTTGTGTTCTGAAAG
Seq C2 exon
GACAACTGTGAGCGCACTGAAAACTCACTGTGCCTTTGGATCAATGAAGCAAAAGACCTTCCCCCAAAGAAGAGATATTATTGTGAGGTCCACCTTGATGGTACACTGTTTGCTCGTACAAGTAGTCGGGCTGTAGGGAAGTCTGTCCAACGCTCCAGCCAGGCAGTTGATGGAGGATCTGGAGCGTTCGGCAGTGGTGGTACTTCAGGAGGTTGCCAGTTGTTCTGGGGTGAATTTTTTGAACTTGACAACTTGCCCCCTGTCACCCAGATCACCCTGCACCTCTTTAAAGGTGAAGACCCCAAGAAGAAACGGCATTCCAGGGACGACGCTACCCTGCATCCTTTAGGAAGTGTAGCTCTCCCTTTGGCAGACATAAAAGGACGTGCCTATCAAGAGAAATGGTATCGAATTGTTGCCTATAAACCCCCGGGAACAAGTACAGCAAAGGATTCGGTTGAACTGCAGGCTTCAATTCGAGTCAAGGCCAAATTCAAGAACCTGAAAATTCTACCTATTGAGAGGTACAAGGAATTTGCAGAGTATGTCACTGTGAATTATGTGGATATGTGTAGCAGCCTTGAACCCCTTCTCAATGTGCGGGAGAAAGAAGAACTAGCTGGAGCACTTGTTCATGTGCTCCAAAGCATTGGGAAAGCTAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000089933:ENSDART00000124643:7
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.303 A=NA C2=0.086
Domain overlap (PFAM):
C1:
PF0016924=PH=PD(17.5=87.9)
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GACCGCTGGATTGAGGACTTG
R:
ACAGCCCGACTACTTGTACGA
Band lengths:
179-272
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]