DreINT0126444 @ danRer10
Intron Retention
Gene
ENSDARG00000071213 | rgl3a
Description
ral guanine nucleotide dissociation stimulator-like 3a [Source:ZFIN;Acc:ZDB-GENE-101130-1]
Coordinates
chr6:7980414-7980698:-
Coord C1 exon
chr6:7980666-7980698
Coord A exon
chr6:7980536-7980665
Coord C2 exon
chr6:7980414-7980535
Length
130 bp
Sequences
Splice sites
5' ss Seq
AAAGTAAGT
5' ss Score
9.72
3' ss Seq
CTGTCCTGTTGTGTGTTTAGGAG
3' ss Score
9.71
Exon sequences
Seq C1 exon
GGAGGCCTCATCAACTTCGAGAAGCGACGAAAA
Seq A exon
GTAAGTAAAAATGCATGAAAAATGAATGCATGTAAATCTGATGTTTTGAGTACTGACCAATGAAGTTTAGCTGCTCACGTGTCAAGATGCATGACTTTTTTTGTGGTGTCCTGTCCTGTTGTGTGTTTAG
Seq C2 exon
GAGTTTGAGATTCTGTCTCTAATCAGGCAGCTGCAGGCGTCATGTGCTCACTACACTCTCCAATCACATCCTCGCATCATTTCCTGGATCAACAGCAGCATGAGTCTTTCTGATCAGAAGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000071213:ENSDART00000151358:13
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.024
Domain overlap (PFAM):
C1:
PF0061714=RasGEF=FE(4.4=100)
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGAGGCCTCATCAACTTCGAG
R:
TCTTCTGATCAGAAAGACTCATGCTG
Band lengths:
154-284
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]