DreINT0126457 @ danRer10
Intron Retention
Gene
ENSDARG00000071213 | rgl3a
Description
ral guanine nucleotide dissociation stimulator-like 3a [Source:ZFIN;Acc:ZDB-GENE-101130-1]
Coordinates
chr6:7985905-7986209:-
Coord C1 exon
chr6:7986106-7986209
Coord A exon
chr6:7985990-7986105
Coord C2 exon
chr6:7985905-7985989
Length
116 bp
Sequences
Splice sites
5' ss Seq
CAGGTAATG
5' ss Score
9.43
3' ss Seq
TGCTTAAAATGTGATTTCAGGGA
3' ss Score
5.01
Exon sequences
Seq C1 exon
GAGTGCCATCAGCTCAGAAACTTCTCCTCTCTAAGAGCCATCCTGTCTGCTCTTCAGTCAAATGCTGTCTACCGGCTGAAGAAAACCTGGGCTGTGATCAGCAG
Seq A exon
GTAATGCTTTCTGATCCACACCACATGTGTTTGTTAAACCAGTACTGCAATGTTTTCTGCTCATATTTAATTTGCACCCCAAATTGTAGAATTAAGTGCTTAAAATGTGATTTCAG
Seq C2 exon
GGAGAGTATGGCTGTCTTTGAACACCTCTGCGACACCTTTCCTGATGAGAACTGTGTGCTGATCAACCGAGAGATCTTAGTAGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000071213:ENSDART00000151358:9
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0061714=RasGEF=FE(14.8=100)
A:
NA
C2:
PF0061714=RasGEF=FE(12.2=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GAGTGCCATCAGCTCAGAAACT
R:
TACTAAGATCTCTCGGTTGATCAGCA
Band lengths:
186-302
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]