DreINT0148148 @ danRer10
Intron Retention
Gene
ENSDARG00000100953 | si:dkeyp-93a5.2
Description
si:dkeyp-93a5.2 [Source:ZFIN;Acc:ZDB-GENE-131127-18]
Coordinates
chr25:24997690-24998647:+
Coord C1 exon
chr25:24997690-24997766
Coord A exon
chr25:24997767-24998460
Coord C2 exon
chr25:24998461-24998647
Length
694 bp
Sequences
Splice sites
5' ss Seq
GAGGTAAGA
5' ss Score
10.06
3' ss Seq
TTTTGTGTCACTGTGAACAGTCT
3' ss Score
6.09
Exon sequences
Seq C1 exon
TACAGAGGACTAAACTATCTGAATAAAGATGTGGAGGAAAACTTGTGTGATTTTGTTGCTGGTGATGTGTGTCAGAG
Seq A exon
GTAAGAACTTGTCTCATTATTTTAAATTAATCTGTGCCTGTATTACATTGAATTAACAAGCTTTCGTTAACATTAAACAGCAAACATTCTTGTTGAATAGTTATTTCTTTTTTTTTTTTTTTTTTCAAATCTTAACTTAATGAAACTAAGTGTCTTGATGCTTTCCCGTAATAATCCCTTCCACACTTTCTCTATTTTCCTGCCAGATTCACTTTCTCAACCAGATGGTCAGTGTCCTTCTCTCTCTTTACATGTTAATTTAAAGTCACTTGGTAATCTATGCAACACAGGTTCATTTGGGAATATGTGCCCAGGGCTACATTTTTGAAAATTGCTAAATATTTGCATGTGAGTGTATATGGCTGCATTTTGTGTGTAAAACAAACACTTTGTGGCGGTACCACTGACTCTGTCTGGTACTCCTGCTGTATAAATGCGATATAATGTCCCATAAATGGTAAAACTTACATCCCTCCATCCCCCCACACTCTACATCTACGTCGCTCACCAACCTCTCTCACGGACCACACAAGAACGATGTTGAGTTTGGCAGAAATGTATACAGTGGCCTGGTAGATTTTTAATGAGAAATGACACGTATGATAAAACATGCCTCGGTAACTCCAATGAGCCTGGGTTGGATTTATTAGAAATGGTCTGGATGTTGTGCACTTTTTTGTGTCACTGTGAACAG
Seq C2 exon
TCTCTCTCTCTCCGTCAGTTTGTGGACGTCCAAATCCCAACTTTAACCCTCGTATTGTGGGTGGTGTGAATGCAACTGAGGGCTCGTGGCCATGGATGGTCAGTCTGCGTAAGAGTGGTGTTCATTTTTGTGGTGGCTCCCTCATCAACAATCAATGGGTGCTGACAGCAGCTCACTGCATCAGTGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000100953:ENSDART00000104453:1
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion (1st CDS intron)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.016
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
PF0008921=Trypsin=PU(18.3=69.8)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Rat
(rn6)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACTATCTGAATAAAGATGTGGAGGA
R:
GCTGTCAGCACCCATTGATTG
Band lengths:
233-927
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]